ArticleACS synthetic biology2019
Engineering the S-Layer of Caulobacter crescentus as a Foundation for Stable, High-Density, 2D Living Materials.
Article in ACS synthetic biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Programming cellular condensates for living materials using an intrinsically disordered protein display platform.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- ALS-ENABLE: creating synergy and opportunity at the Advanced Light Source synchrotron structural biology beamlines.Journal of synchrotron radiation · 2025Article
- Bacterial Species in Engineered Living Materials: Strategies and Future Directions.Microbial biotechnology · 2025Review
- Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus.Nature communications · 2024Article
- Article
- Population-level heterogeneity complicates utilization ofmicroPublication biology · 2024Article
- Sneaking in SpyCatcher using cell penetrating peptides forNanotechnology · 2023Article
- Interdigitated immunoglobulin arrays form the hyperstable surface layer of the extremophilic bacteriumProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Living electronics: A catalogue of engineered living electronic components.Microbial biotechnology · 2023Review
- Synchronized Swarmers and Sticky Stalks: Caulobacter crescentus as a Model for Bacterial Cell Biology.Journal of bacteriology · 2023Review
- Emerging enzyme surface display systems for waste resource recovery.Environmental microbiology · 2023Review
- A de novo matrix for macroscopic living materials from bacteria.Nature communications · 2022Article
- In vitro investigation of protein assembly by combined microscopy and infrared spectroscopy at the nanometer scale.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly.Nature communications · 2022Article
- Engineering living and regenerative fungal-bacterial biocomposite structures.Nature materials · 2022Article
- High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells.Structure (London, England : 1993) · 2022Article
- Complete atomic structure of a native archaeal cell surface.Cell reports · 2021Article
- Synthetic biology as driver for the biologization of materials sciences.Materials today. Bio · 2021Review
- Robust Self-Regeneratable Stiff Living Materials Fabricated from Microbial Cells.Advanced functional materials · 2021Article
- Molecular Logic of Prokaryotic Surface Layer Structures.Trends in microbiology · 2021Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Materials synthesized by organisms, such as bones and wood, combine the ability to self-repair with remarkable mechanical properties. This multifunctionality arises from the presence of living cells within the material and hierarchical assembly of different components across nanometer to micron scales. While creating engineered analogues of these natural materials is of growing interest, our ability to hierarchically order materials using living cells largely relies on engineered 1D protein filaments. Here, we lay the foundation for bottom-up assembly of engineered living material composites in 2D along the cell body using a synthetic biology approach. We engineer the paracrystalline surface-layer (S-layer) of Caulobacter crescentus to display SpyTag peptides that form irreversible isopeptide bonds to SpyCatcher-modified proteins, nanocrystals, and biopolymers on the extracellular surface. Using flow cytometry and confocal microscopy, we show that attachment of these materials to the cell surface is uniform, specific, and covalent, and its density can be controlled on the basis of the insertion location within the S-layer protein, RsaA. Moreover, we leverage the irreversible nature of this attachment to demonstrate via SDS-PAGE that the engineered S-layer can display a high density of materials, reaching 1 attachment site per 288 nm
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Registered trials
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