ArticleNanomaterials (Basel, Switzerland)2018
Characterization of Nanoparticle Intestinal Transport Using an In Vitro Co-Culture Model.
Article in Nanomaterials (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Application of 3D Cell Culture Techniques in Nanotoxicology: How Far Are We?Stem cell reviews and reports · 2026Review
- In Vitro and Ex Vivo Models to Study Molecular Trafficking Across the Human Intestinal Barrier.International journal of molecular sciences · 2025Review
- Transport Mechanisms of 2D Nanoparticles across a Human Follicle-Associated Epithelium Model.ACS omega · 2025Article
- Nanoparticle Uptake and Crossing by Human In Vitro Models of Intestinal Barriers: A Scoping Review.Nanomaterials (Basel, Switzerland) · 2025Review
- Three-Dimensional Lymphatics-on-a-Chip Reveals Distinct, Size-Dependent Nanoparticle Transport Mechanisms in Lymphatic Drug Delivery.ACS biomaterials science & engineering · 2024Article
- Size-Dependent Internalization of Microplastics and Nanoplastics Using In Vitro Model of the Human Intestine-Contribution of Each Cell in the Tri-Culture Models.Nanomaterials (Basel, Switzerland) · 2024Article
- Nanoparticles in Medicine: Current Status in Cancer Treatment.International journal of molecular sciences · 2023Review
- Fermentation Supernatant of Elderly Feces with Inulin and Partially Hydrolyzed Guar Gum Maintains the Barrier of Inflammation-Induced Caco-2/HT29-MTX-E12 Co-Cultured Cells.Journal of agricultural and food chemistry · 2023Article
- Interferon gamma upregulates the cytokine receptors IFNGR1 and TNFRSF1A in HT-29-MTX E12 cells.Cytokine · 2022Article
- Surfactant-Free Glibenclamide Nanoparticles: Formulation, Characterization and Evaluation of Interactions with Biological Barriers.Pharmaceutical research · 2021Article
- Intestinal and hepatic effects of iron oxide nanoparticles.Archives of toxicology · 2021Article
- Caco-2/HT29-MTX co-cultured cells as a model for studying physiological properties and toxin-induced effects on intestinal cells.PloS one · 2021Article
- The Role of Metal Oxide Nanoparticles,Environmental science. Nano · 2020Article
- Review
- Selected Papers from the 1st International Online Conference on Nanomaterials.Nanomaterials (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We aimed to obtain a tunable intestinal model and study the transport of different types of nanoparticles. Caco-2/HT29-MTX co-cultures of different seeding ratios (7:3 and 5:5), cultured on Transwell® systems, were exposed to non-cytotoxic concentration levels (20 μg/mL) of silicon quantum dots and iron oxide (α-Fe₂O₃) nanoparticles. Transepithelial electric resistance was measured before and after exposure, and permeability was assessed via the paracellular marker Lucifer Yellow. At regular intervals during the 3 h transport study, samples were collected from the basolateral compartments for the detection and quantitative testing of nanoparticles. Cell morphology characterization was done using phalloidin-FITC/DAPI labeling, and Alcian Blue/eosin staining was performed on insert cross-sections in order to compare the intestinal models and evaluate the production of mucins. Morphological alterations of the Caco-2/HT29-MTX (7:3 ratio) co-cultures were observed at the end of the transport study compared with the controls. The nanoparticle suspensions tested did not diffuse across the intestinal model and were not detected in the receiving compartments, probably due to their tendency to precipitate at the monolayer surface level and form visible aggregates. These preliminary results indicate the need for further nanoparticle functionalization in order to appropriately assess intestinal absorption in vitro.
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Registered trials
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