Evidence map›Paper›PMID 30576862›Full record

ArticleActa biomaterialia2019

3D printed micro-scale force gauge arrays to improve human cardiac tissue maturation and enable high throughput drug testing.

Xuanyi Ma, Sukriti Dewan, Justin Liu, Min Tang, Kathleen L Miller, Claire Yu, Natalie Lawrence, Andrew D McCulloch, Shaochen Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 1 pooled it
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.

  1. Pooled it
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  11. Article
  12. Article
  13. Optimization of 3D printing andFrontiers in bioengineering and biotechnology · 2023
    Article
  14. Review
  15. Article
  16. High throughput screening system for engineered cardiac tissues.Frontiers in bioengineering and biotechnology · 2023
    Article
  17. Review
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  19. Recent advances in bioprinting technologies for engineering cardiac tissue.Materials science & engineering. C, Materials for biological applications · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Xuanyi MaDepartment of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Sukriti DewanDepartment of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Justin LiuDepartment of Materials Science and Engineering Program, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Min TangDepartment of NanoEngineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Kathleen L MillerDepartment of NanoEngineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Claire YuDepartment of NanoEngineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Natalie LawrenceDepartment of NanoEngineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Andrew D McCullochDepartment of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA; Department of Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Shaochen ChenDepartment of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA; Department of Materials Science and Engineering Program, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA; Department of NanoEngineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA; Chemical Engineering Program, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA. Electronic address: chen168@eng.ucsd.edu.
University of California San Diego · US

Funding

U of Calif, San Diego Neuroscience Microscopy ImagingP30NS047101 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G, ZHENG, BINHAI · 2003 to 2022
$9.0M
Rapid 3D bioprinting of biomimetic vascularized tissue constructsR01EB021857 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN, DOKMECI, MEHMET REMZI · 2016 to 2019
$2.1M
Systems Biology of Hypertrophic Heart Disease from Molecular Pathways to Organ SystemR01HL137100 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MCCULLOCH, ANDREW D. · 2017 to 2020
$2.0M
3D Printed Scaffolds for Primate Spinal Cord InjuryR03NS104972 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN, TUSZYNSKI, MARK H. · 2017 to 2018
$154k
NHLBI NIH HHS R01 HL137100NIBIB NIH HHS R01 EB021857NINDS NIH HHS P30 NS047101NINDS NIH HHS R03 NS104972
6 · The paper itself

Abstract

Human induced pluripotent stem cell - derived cardiomyocytes (iPSC-CMs) are regarded as a promising cell source for establishing in-vitro personalized cardiac tissue models and developing therapeutics. However, analyzing cardiac force and drug response using mature human iPSC-CMs in a high-throughput format still remains a great challenge. Here we describe a rapid light-based 3D printing system for fabricating micro-scale force gauge arrays suitable for 24-well and 96-well plates that enable scalable tissue formation and measurement of cardiac force generation in human iPSC-CMs. We demonstrate consistent tissue band formation around the force gauge pillars with aligned sarcomeres. Among the different maturation treatment protocols we explored, 3D aligned cultures on force gauge arrays with in-culture pacing produced the highest expression of mature cardiac marker genes. We further demonstrated the utility of these micro-tissues to develop significantly increased contractile forces in response to treatment with isoproterenol, levosimendan, and omecamtiv mecarbil. Overall, this new 3D printing system allows for high flexibility in force gauge design and can be optimized to achieve miniaturization and promote cardiac tissue maturation with great potential for high-throughput in-vitro drug screening applications. STATEMENT OF SIGNIFICANCE: The application of iPSC-derived cardiac tissues in translatable drug screening is currently limited by the challenges in forming mature cardiac tissue and analyzing cardiac forces in a high-throughput format. We demonstrate the use of a rapid light-based 3D printing system to build a micro-scale force gauge array that enables scalable cardiac tissue formation from iPSC-CMs and measurement of contractile force development. With the capability to provide great flexibility over force gauge design as well as optimization to achieve miniaturization, our 3D printing system serves as a promising tool to build cardiac tissues for high-throughput in-vitro drug screening applications.

Indexed as

Printing, Three-DimensionalAdultAnimalsBiomechanical PhenomenaDrug Evaluation, PreclinicalHigh-Throughput Screening AssaysHumansInduced Pluripotent Stem CellsMaleMiceMyocardium3D printingCardiac force measurementDrug testingiPSC-derived cardiomyocytes

Identifiers

PMID30576862
PMCPMC6584548
OpenAlexW2904356888

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.