ArticlePloS one2018
A user-friendly platform for yeast two-hybrid library screening using next generation sequencing.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
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- NGPINT V3: a containerized orchestration Python software for discovery of next-generation protein-protein interactions.Bioinformatics (Oxford, England) · 2025Article
- Workflow to Select Functional Promoter DNA Baits and Screen Arrayed Gene Libraries in Yeast.Current protocols · 2024Article
- Massively parallel measurement of protein-protein interactions by sequencing using MP3-seq.Nature chemical biology · 2024Article
- Genome‑wide analysis of the GT8 gene family in apple and functional identification of MhGolS2 in saline-alkali tolerance.Plant molecular biology · 2024Article
- Powdery mildew effectors AVRMolecular plant pathology · 2024Article
- Exploring the potential role of fourFrontiers in plant science · 2024Article
- Characterization of Arbuscular Mycorrhizal Effector Proteins.International journal of molecular sciences · 2023Review
- Determination of Key Components in theInsects · 2023Article
- From prediction to function: Current practices and challenges towards the functional characterization of type III effectors.Frontiers in microbiology · 2023Review
- Next-Generation Yeast Two-Hybrid Screening to Discover Protein-Protein Interactions.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Cataloguing Protein Complexes In Planta Using TurboID-Catalyzed Proximity Labeling.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Fine Mapping of a Vigor QTL in Chickpea (Frontiers in plant science · 2022Article
- Unraveling Protein Interactions between the Temperate Virus Bam35 and ItsInternational journal of molecular sciences · 2021Article
- Transcription Factors Interact with ABA through Gene Expression and Signaling Pathways to Mitigate Drought and Salinity Stress.Biomolecules · 2021Review
- Advances and perspectives in discovery and functional analysis of small secreted proteins in plants.Horticulture research · 2021Review
- Visualizing protein-protein interactions in plants by rapamycin-dependent delocalization.The Plant cell · 2021Article
- Next-generation yeast-two-hybrid analysis with Y2H-SCORES identifies novel interactors of the MLA immune receptor.PLoS computational biology · 2021Article
- Determination of conifer age biomarker DAL1 interactome using Y2H-seq.Forestry research · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yeast two-hybrid (Y2H) is a well-established genetics-based system that uses yeast to selectively display binary protein-protein interactions (PPIs). To meet the current need to unravel complex PPI networks, several adaptations have been made to establish medium- to high-throughput Y2H screening platforms, with several having successfully incorporated the use of the next-generation sequencing (NGS) technology to increase the scale and sensitivity of the method. However, these have been to date mainly restricted to the use of fully annotated custom-made open reading frame (ORF) libraries and subject to complex downstream data processing. Here, a streamlined Y2H library screening strategy, based on integration of Y2H with NGS, called Y2H-seq, was developed, which allows efficient and reliable screening of Y2H cDNA libraries. To generate proof of concept, the method was applied to screen for interaction partners of two key components of the jasmonate signaling machinery in the model plant Arabidopsis thaliana, resulting in the identification of several previously reported as well as hitherto unknown interactors. Our Y2H-seq method offers a user-friendly, specific and sensitive screening method that allows identification of PPIs without prior knowledge of the organism's ORFs, thereby extending the method to organisms of which the genome has not entirely been annotated yet. The quantitative NGS readout allows to increase genome coverage, thereby overcoming some of the bottlenecks of current Y2H technologies, which will further strengthen the value of the Y2H technology as a discovery platform.
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