ArticleScientific reports2018
Corynebacterium Cell Factory Design and Culture Process Optimization for Muconic Acid Biosynthesis.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Engineering of Corynebacterium glutamicum for biosynthesis of the pharmaceutically active N-acetyltyramine: establishing and optimizing de novo production.Journal of biological engineering · 2026Article
- Multiscale metabolic engineering in biological lignin valorization.Innovation (Cambridge (Mass.)) · 2025Review
- Recent advances of muconic acid production using microbial synthetic biology.Archives of microbiology · 2025Review
- Hierarchical metabolic engineering for rewiring cellular metabolism.FEMS microbiology reviews · 2025Review
- Enhancement Strategy for Protocatechuic Acid Production UsingInternational journal of molecular sciences · 2025Article
- Metabolic Engineering and Process Intensification for Muconic Acid Production UsingInternational journal of molecular sciences · 2024Article
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- Metabolic Engineering ofMolecules (Basel, Switzerland) · 2024Review
- Biosensors for the detection of chorismate and cis,cis-muconic acid in Corynebacterium glutamicum.Journal of industrial microbiology & biotechnology · 2024Article
- Exploring the secretome ofFrontiers in bioengineering and biotechnology · 2024Article
- CRISPR-Driven Genome Engineering for Chorismate- and Anthranilate-AccumulatingJournal of microbiology and biotechnology · 2023Article
- Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering.Nature communications · 2022Article
- Artificial cell factory design for shikimate production in Escherichia coli.Journal of industrial microbiology & biotechnology · 2021Article
- Shikimate Metabolic Pathway Engineering inJournal of microbiology and biotechnology · 2021Article
- Catechol 1,2-Dioxygenase FromFrontiers in bioengineering and biotechnology · 2021Article
- Recent Advances in Microbial Production ofBiomolecules · 2020Review
- Cell Factory Design and Culture Process Optimization for Dehydroshikimate Biosynthesis inFrontiers in bioengineering and biotechnology · 2019Article
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
Abstract
Muconic acid (MA) is a valuable compound for adipic acid production, which is a precursor for the synthesis of various polymers such as plastics, coatings, and nylons. Although MA biosynthesis has been previously reported in several bacteria, the engineered strains were not satisfactory owing to low MA titers. Here, we generated an engineered Corynebacterium cell factory to produce a high titer of MA through 3-dehydroshikimate (DHS) conversion to MA, with heterologous expression of foreign protocatechuate (PCA) decarboxylase genes. To accumulate key intermediates in the MA biosynthetic pathway, aroE (shikimate dehydrogenase gene), pcaG/H (PCA dioxygenase alpha/beta subunit genes) and catB (chloromuconate cycloisomerase gene) were disrupted. To accomplish the conversion of PCA to catechol (CA), a step that is absent in Corynebacterium, a codon-optimized heterologous PCA decarboxylase gene was expressed as a single operon under the strong promoter in a aroE-pcaG/H-catB triple knock-out Corynebacterium strain. This redesigned Corynebacterium, grown in an optimized medium, produced about 38 g/L MA and 54 g/L MA in 7-L and 50-L fed-batch fermentations, respectively. These results show highest levels of MA production demonstrated in Corynebacterium, suggesting that the rational cell factory design of MA biosynthesis could be an alternative way to complement petrochemical-based chemical processes.
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