ArticleNature communications2018
Synthetic mammalian pattern formation driven by differential diffusivity of Nodal and Lefty.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Fundamental limits on symmetry breaking by Turing-like activator-inhibitor mechanisms.Development (Cambridge, England) · 2026Article
- A novel reaction-diffusion architecture for engineering self-organized patterns in mammalian cells.bioRxiv : the preprint server for biology · 2026Article
- Left-Right Determination Factor 2 (LEFTY2) Is an Aqueous Humor Biomarker for Exfoliation Glaucoma.Translational vision science & technology · 2026Article
- Engineering development: From the repressilator and toggle switch to synthetic developmental biology.Developmental biology · 2025Review
- Designer mammalian living materials through genetic engineering.Bioactive materials · 2025Review
- Optimal network sizes for most robust Turing patterns.Scientific reports · 2025Article
- Live imaging of paracrine signaling: Advances in visualization and tracking techniques.Cell structure and function · 2025Review
- Synthetically programming natural cell-cell communication pathways for tissue engineering.Current opinion in biomedical engineering · 2024Article
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- Templated Pluripotent Stem Cell Differentiation via Substratum-Guided Artificial Signaling.ACS biomaterials science & engineering · 2024Article
- Unraveling biochemical spatial patterns: Machine learning approaches to the inverse problem of stationary Turing patterns.iScience · 2024Article
- Review
- Understanding and manipulating extracellular behaviors of Wnt ligands.In vitro cellular & developmental biology. Animal · 2024Review
- Reconstitution of morphogen shuttling circuits.Science advances · 2023Article
- SyNPL: Synthetic Notch pluripotent cell lines to monitor and manipulate cell interactions in vitro and in vivo.Development (Cambridge, England) · 2022Article
- Synthetic spatial patterning in bacteria: advances based on novel diffusible signals.Microbial biotechnology · 2022Review
- Advances and challenges in programming pattern formation using living cells.Current opinion in chemical biology · 2022Review
- Engineering multiscale structural orders for high-fidelity embryoids and organoids.Cell stem cell · 2022Review
- Synthetic Morphogenesis: introducing IEEE journal readers to programming living mammalian cells to make structures.Proceedings of the IEEE. Institute of Electrical and Electronics Engineers · 2022Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A synthetic mammalian reaction-diffusion pattern has yet to be created, and Nodal-Lefty signaling has been proposed to meet conditions for pattern formation: Nodal is a short-range activator whereas Lefty is a long-range inhibitor. However, this pattern forming possibility has never been directly tested, and the underlying mechanisms of differential diffusivity of Nodal and Lefty remain unclear. Here, through a combination of synthetic and theoretical approaches, we show that a reconstituted Nodal-Lefty network in mammalian cells spontaneously gives rise to a pattern. Surprisingly, extracellular Nodal is confined underneath the cells, resulting in a narrow distribution compared with Lefty. The short-range distribution requires the finger 1 domain of Nodal, and transplantation of the finger 1 domain into Lefty shortens the distribution of Lefty, successfully preventing pattern formation. These results indicate that the differences in localization and domain structures between Nodal and Lefty, combined with the activator-inhibitor topology, are sufficient for reaction-diffusion patterning.
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