Evidence map›Paper›PMID 30574188›Full record

ArticleBiotechnology for biofuels2018

Comparative transcriptome and metabolome analysis suggests bottlenecks that limit seed and oil yields in transgenic

Hesham M Abdullah, Sudesh Chhikara, Parisa Akbari, Danny J Schnell, Ashwani Pareek, Om Parkash Dhankher

Open access · goldAbstract read
In one paragraph

Article in Biotechnology for biofuels, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 26 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 3 countries.

Hesham M AbdullahStockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003 USA.
Sudesh ChhikaraStockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003 USA.
Parisa AkbariStockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003 USA.
Danny J Schnell3Department of Plant Biology, Michigan State University, East Lansing, MI 48824 USA.
Ashwani Pareek4Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 100067 India.
Om Parkash DhankherStockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003 USA.ORCID 0000-0003-0737-6783
University of Massachusetts Amherst · USAl-Azhar University · EGJawaharlal Nehru University · INMichigan State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

background

resultsTranscriptomic analysis revealed approximately 2518 and 3136 transcripts differentially regulated at significant levels in DGAT1 and GPD1 transgenics, respectively. These transcripts were found to be involved in various functional categories, including alternative metabolic routes in fatty acid synthesis, TAG assembly, and TAG degradation. We quantified the relative contents of over 240 metabolites. Our results indicate major metabolic switches in transgenic seeds associated with significant changes in the levels of glycerolipids, amino acids, sugars, and organic acids, especially the TCA cycle and glycolysis intermediates.

conclusionsFrom the transcriptomic and metabolomic analysis of DGAT1, GPD1 and DGAT1 + GPD1 expressing lines of

Indexed as

Camelina sativaDiacylglycerol acyltransferaseGlycerol-3-phosphate dehydrogenaseMetabolic engineeringMetabolome profilingOilseedRNA-SeqTAG biosynthesis

Identifiers

PMID30574188
PMCPMC6299664
OpenAlexW2904904275

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.