ArticleScientific reports2018
Modulation of benzylisoquinoline alkaloid biosynthesis by overexpression berberine bridge enzyme in Macleaya cordata.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 25 citations in OpenAlex.
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- Dose-dependent effects of melatonin on berberine production, antioxidant activity, and metabolite profiles in Berberis vulgaris L. suspension cultures.Scientific reports · 2025Article
- Biotechnological Advances in Sanguinarine and Chelerythrine Production from Plume Poppy (Plants (Basel, Switzerland) · 2025Review
- Clinicopathological features and renal outcomes in patients with IgA nephropathy secondary to psoriasis.Clinical and experimental medicine · 2025Article
- Comparative transcriptome reveals lignin biosynthesis being the key molecular pathway regulating oilseed rape growth treated by SiOJournal of plant research · 2025Article
- Convergent evolution of berberine biosynthesis.Science advances · 2024Article
- Recent Applications of Protoberberines as Privileged Starting Materials for the Development of Novel Broad-Spectrum Antiviral Agents: A Concise Review (2017-2023).ACS pharmacology & translational science · 2024Review
- Recent topics in X-ray fluorescence spectrometry.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- Isolation and Characterization of the Genes Involved in the Berberine Synthesis Pathway in Asian Blue Cohosh,Plants (Basel, Switzerland) · 2023Article
- Article
- Daurisoline Inhibiting Tumor Angiogenesis and Epithelial-Mesenchymal Transition in Bladder Cancer by Mediating HAKAI Protein Stability.Iranian journal of pharmaceutical research : IJPR · 2022Article
- Application of metabolic engineering to enhance the content of alkaloids in medicinal plants.Metabolic engineering communications · 2022Review
- Effects of codon optimization, N-terminal truncation and gene dose on the heterologous expression of berberine bridge enzyme.World journal of microbiology & biotechnology · 2022Article
- Sanguinarine protects against indomethacin-induced small intestine injury in rats by regulating the Nrf2/NF-κB pathways.Frontiers in pharmacology · 2022Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Macleaya cordata produces a variety of benzylisoquinoline alkaloids (BIAs), such as sanguinarine, protopine, and berberine, which are potential anticancer drugs and natural growth promoters. The genes encoding the berberine bridge enzyme (BBE) were isolated from M. cordata and Papaver somniferum, and then the two genes were overexpressed in M. cordata. Through liquid chromatography with triple-quadrupole mass spectrometry analysis, it was determined that McBBE-OX caused higher levels of (S)-norcoclaurine, (S)-coclaurine, (S)-N-cis-methylcoclaurine, (S)-reticuline, (S)-tetrahydrocolumbamine, (S)-tetrahydroberberine, (S)-cheilanthifoline, and (S)-scoulerine than PsBBE-OX, empty vector or control treatments. qRT-PCR analysis demonstrated that the introduced genes in the transgenic lines were all highly expressed. However, the levels of sanguinarine (SAN) and chelerythrine (CHE) in all the transgenic lines were slightly lower than those in the wild-type lines, possibly because the overexpression of McBBE causes feedback-inhibition. This is the first report on the overexpression of potential key genes in M. cordata, and the findings are important for the design of metabolic engineering strategies that target BIAs biosynthesis.
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