ArticleBMC veterinary research2018
Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis.
Article in BMC veterinary research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Synergistic Integration of Recombinase Polymerase Amplification and CRISPR-Cas12a Enables High-Sensitivity Detection of Mycoplasma bovis.MicrobiologyOpen · 2026Article
- Evaluation of recombinase polymerase amplification assays for targeted detection of bovine respiratory disease bacterial pathogens and antimicrobial-resistance genes in feedlot calves.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2026Article
- Bovine Respiratory Mycoplasmas and the Commensal-Pathogen Continuum: A Systematic Review of Vaccines and Diagnostic Approaches.Animals : an open access journal from MDPI · 2026Review
- Survey of bacteria associated with septic arthritis in beef feedlot cattle.Applied and environmental microbiology · 2026Article
- Article
- Development of polymerase chain reaction-lateral flow dipstick assay for detection of Mycoplasma bovis in cattle.BMC veterinary research · 2024Article
- Laboratory tools for the direct detection of bacterial respiratory infections and antimicrobial resistance: a scoping review.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Point-of-care potentials of lateral flow-based field screening forBiology methods & protocols · 2024Review
- Establishment of a Real-Time Recombinase Polymerase Amplification for Rapid Detection of PathogenicPathogens (Basel, Switzerland) · 2023Article
- Microfluidic chip and isothermal amplification technologies for the detection of pathogenic nucleic acid.Journal of biological engineering · 2022Review
- Development and evaluation of recombinase polymerase amplification combined with lateral flow dipstick assays for co-detection of epizootic haemorrhagic disease virus and the Palyam serogroup virus.BMC veterinary research · 2021Article
- Direct and Rapid Detection ofFrontiers in cellular and infection microbiology · 2021Article
- Development and application of a colloidal carbon test strip for the detection of antibodies against Mycoplasma bovis.World journal of microbiology & biotechnology · 2020Article
- Induction of the Unfolded Protein Response during Bovine Alphaherpesvirus 1 Infection.Viruses · 2020Article
- Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs.Molecules (Basel, Switzerland) · 2020Review
- Review
- Detection of bovine viral diarrhea virus genotype 1 in aerosol by a real time RT-PCR assay.BMC veterinary research · 2020Article
- Fast, simple and highly specific molecular detection of Vibrio alginolyticus pathogenic strains using a visualized isothermal amplification method.BMC veterinary research · 2020Article
- Alteration of cell junctions during viral infection.Thoracic cancer · 2020Review
- Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus.Journal of virological methods · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundMycoplasma bovis (M. bovis) is a major etiological agent of bovine mycoplasmosis around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The recombinase polymerase amplification (RPA) technique has become a promising isothermal DNA amplify assay for use in rapid and low-resource diagnostics.
resultsHere, a method for specific detection of M. bovis DNA was established, which was RPA combined with lateral flow dipstick (LFD). First, the analytical specificity and sensitivity of the RPA primer and LF-probe sets were evaluated. The assay successfully detected M. bovis DNA in 30 min at 39 °C, with detection limit of 20 copies per reaction, which it was compared the real-time quantitative PCR (qPCR) assay. This method was specific because it did not detect a selection of other bacterial pathogens in cattle. Both qPCR and RPA-LFD assays were used to detect M. bovis 442 field samples from 42 different dairy farms in Shandong Province of China, also the established RPA-LFD assay obtained 99.00% sensitivity, 95.61% specificity, and 0.902 kappa coefficient compared with the qPCR.
conclusionsTo the author's knowledge, this is the first report using an RPA-FLD assay to visualise and detect M. bovis. Comparative analysis with qPCR indicates the potential of this assay for rapid diagnosis of bovine mycoplasmosis in resource limited settings.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.