Evidence map›Paper›PMID 30572833›Full record

ArticleBMC infectious diseases2018

Successful infection of BALB/c mice by a swine hepatitis E virus clone constructed with reverse genetics.

Wenhai Yu, Chenchen Yang, Xianhui Hao, Tianwu Ma, Fen Huang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC infectious diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Wenhai YuInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, 935 Jiaoling Road, Kunming, China.
Chenchen YangMedical School, Kunming University of Science and Technology, 727 Jingming Road, Kunming, China.
Xianhui HaoMedical School, Kunming University of Science and Technology, 727 Jingming Road, Kunming, China.
Tianwu MaMedical School, Kunming University of Science and Technology, 727 Jingming Road, Kunming, China.
Fen HuangMedical School, Kunming University of Science and Technology, 727 Jingming Road, Kunming, China. huangfen6789@163.com.ORCID http://orcid.org/0000-0001-9147-2537
Kunming University of Science and Technology · CNChinese Academy of Medical Sciences & Peking Union Medical College · CN

Funding

Fundamental Research Funds for the Central Universities 20162X310179-3National Natural Science Foundation of China 31360619National Natural Science Foundation of China 81660338Natural Science Foundation of Yunnan Province 2017FA036Natural Science Foundation of Yunnan Province 2018FB132PUMC Youth Fund 2017310038
6 · The paper itself

Abstract

backgroundHepatitis E virus (HEV) is a leading cause of hepatitis worldwide. However, its infection biology and pathogenesis remain largely elusive. Furthermore, no proven medication is available for treating hepatitis E. Robust experimental models are urgently required to advance the research of HEV infection. Because of the lacking of a sophisticated small animal model, this study aimed to establish a mouse model of HEV infection.

methodsWe constructed a full-length swine HEV cDNA clone of genotype 4 (named as pGEM-HEV) by reverse genetics approach. And we inoculated with HEV RNA in BALB/c mice to establish small animal model for HEV infection and pathogenesis studies.

resultsThe capped RNA transcripts of pGEM-HEV prepared in vitro were replication-competent in HepG2 cells. Importantly, BALB/c mice intravenously inoculated with RNA transcripts of pGEM-HEV developed an active infection as shown by shedding viruses in feces, detectable negative strand of HEV in the liver, spleen and kidney, and causing liver inflammation.

conclusionIn this study, we successfully established of BALB/c mice-based small animal model for HEV provides an opportunity to further understand HEV pathogenesis and to develop effective antiviral medications.

Indexed as

Disease Models, AnimalMice, Inbred BALB CAnimalsCell Line, TumorCloning, OrganismFemaleHepatitis EHepatitis E virusHep G2 CellsHumansMiceReverse GeneticsRNA, ViralSwineSwine DiseasesVirus SheddingRNA, ViralBALB/c miceHepatitis E virusInfectious cDNA cloneInfectivity

Identifiers

PMID30572833
PMCPMC6302442
OpenAlexW2904322402

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.