ArticleAnalytical chemistry2019
High-Throughput Automated Micro-permethylation for Glycan Structure Analysis.
Article in Analytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Navigating the glycomics landscape with CE-MS: advances in sample preparation and analytical strategies.The Analyst · 2026Review
- High-sensitive glycomics using seGRAP-mass spectrometry uncovers a conserved N-glycome in single human oocytes.Nature communications · 2026Article
- Protocol for simultaneous profiling of N- and O-glycans on glycoproteins using a one-pot format.STAR protocols · 2026Article
- Development, validation, and assessment of a reliable and white method for the analysis of water-based perfumes by pipette-tip micro-solid-phase extraction combined with dual detection gas chromatography.Analytical and bioanalytical chemistry · 2025Article
- Recent advances in analytical methods and bioinformatic tools for quantitative glycomics.Analytical and bioanalytical chemistry · 2025Review
- Efficient derivatization-free monitoring of glycosyltransferase reactions via flow injection analysis-mass spectrometry for rapid sugar analytics.Analytical and bioanalytical chemistry · 2024Article
- Toward Automatic Inference of Glycan Linkages Using MSJournal of the American Society for Mass Spectrometry · 2023Article
- Site specific N- and O-glycosylation mapping of the spike proteins of SARS-CoV-2 variants of concern.Scientific reports · 2023Article
- Site Specific N- and O-glycosylation mapping of the Spike Proteins of SARS-CoV-2 Variants of Concern.Research square · 2022Article
- LC-MS/MS Isomeric Profiling ofBiomolecules · 2022Article
- High sensitivity glycomics in biomedicine.Mass spectrometry reviews · 2022Review
- Developments and perspectives in high-throughput protein glycomics: enabling the analysis of thousands of samples.Glycobiology · 2022Article
- Article
- Automation of Immunoglobulin Glycosylation Analysis.Experientia supplementum (2012) · 2021Review
- Simplifying Glycan Profiling through a High-Throughput Micropermethylation Strategy.SLAS technology · 2020Article
- The role of the mucin-glycan foragingGut microbesArticle
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Permethylation is an essential technique for the detailed structural characterization of glycans by mass spectrometry. However, it requires specialized skills and equipment and is labor-intensive. While this limits glycan analysis to a handful of experts, the increasing awareness of the tremendous importance of glycans in physiological processes of health and disease has drastically raised the demand for detailed structural characterization of glycans. We have developed a simple microplate permethylation method that relies upon solid phase extraction using C18 tips to purify the permethylated glycans. The procedure is easy to perform, making it accessible to nonexperts, and fast, promising to accelerate glycan research. A 96-well plate of released glycan samples can be permethylated in less than an hour. The procedure can be carried out without discontinuous steps in an automatic pipet and integrated seamlessly with glycan release and mass spectrometry.
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Registered trials
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