Evidence map›Paper›PMID 30572283›Full record

ArticleBiomaterials2019

Spatiotemporally-controlled transgene expression in hydroxyapatite-fibrin composite scaffolds using high intensity focused ultrasound.

Alexander Moncion, Jennifer N Harmon, Yan Li, Sam Natla, Easton C Farrell, Oliver D Kripfgans, Jan P Stegemann, Francisco M Martín-Saavedra, Nuria Vilaboa, Renny T Franceschi and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 23 citations in OpenAlex.

  1. Ultrasound-responsive composite hydrogels: Design rules for spatiotemporally controlled drug delivery.Journal of controlled release : official journal of the Controlled Release Society · 2026
    Review
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  5. CaBioactive materials · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Alexander MoncionApplied Physics Program, University of Michigan, Ann Arbor, MI, USA; Department of Radiology, University of Michigan Health System, Ann Arbor, MI, USA.
Jennifer N HarmonDepartment of Radiology, University of Michigan Health System, Ann Arbor, MI, USA.
Yan LiDepartment of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI, USA.
Sam NatlaDepartment of Radiology, University of Michigan Health System, Ann Arbor, MI, USA.
Easton C FarrellDepartment of Radiology, University of Michigan Health System, Ann Arbor, MI, USA.
Oliver D KripfgansApplied Physics Program, University of Michigan, Ann Arbor, MI, USA; Department of Radiology, University of Michigan Health System, Ann Arbor, MI, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Jan P StegemannDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Francisco M Martín-SaavedraHospital Universitario La Paz-IdiPAZ, Madrid, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Madrid, Spain.
Nuria VilaboaHospital Universitario La Paz-IdiPAZ, Madrid, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Madrid, Spain.
Renny T FranceschiDepartment of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA; Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI, USA.
Mario L FabiilliApplied Physics Program, University of Michigan, Ann Arbor, MI, USA; Department of Radiology, University of Michigan Health System, Ann Arbor, MI, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA. Electronic address: mfabiill@umich.edu.
Michigan Medicine · USUniversity of Michigan–Ann Arbor · USBiomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine · ESHospital Universitario La Paz · ES

Funding

Structure, Composition, & Histology Core - Core BP30AR069620 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KARL J JEPSEN · 2016 to 2026
$8.4M
Patterning of Bone Regeneration via Ultrasound Activation of Heat Shock-Responsive Gene SwitchesR21AR072336 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FABIILLI, MARIO LEONARDO, FRANCESCHI, RENNY THEODORE · 2018 to 2019
$378k
NIAMS NIH HHS P30 AR069620NIAMS NIH HHS R21 AR072336
6 · The paper itself

Abstract

Conventional tissue engineering approaches rely on scaffold-based delivery of exogenous proteins, genes, and/or cells to stimulate regeneration via growth factor signaling. However, scaffold-based approaches do not allow active control of dose, timing, or spatial localization of a delivered growth factor once the scaffold is implanted, yet these are all crucial parameters in promoting tissue regeneration. To address this limitation, we developed a stable cell line containing a heat-activated and rapamycin-dependent gene expression system. In this study, we investigate how high intensity focused ultrasound (HIFU) can spatiotemporally control firefly luciferase (fLuc) transgene activity both in vitro and in vivo by the tightly controlled generation of hyperthermia. Cells were incorporated into composite scaffolds containing fibrin and hydroxyapatite particles, which yielded significant increases in acoustic attenuation and heating in response to HIFU compared to fibrin alone. Using 2.5 MHz HIFU, transgene activation was observed at acoustic intensities of 201 W/cm

Indexed as

Gene ExpressionTransgenesAnimalsBiocompatible MaterialsCell LineDurapatiteFemaleFibrinHigh-Intensity Focused Ultrasound AblationLuciferases, FireflyMiceTissue EngineeringTissue ScaffoldsBiocompatible MaterialsDurapatiteFibrinLuciferases, FireflyFibrinGene therapyHigh intensity focused ultrasoundHydrogelHydroxyapatiteHyperthermia

Identifiers

PMID30572283
PMCPMC6339574
OpenAlexW2904355355

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.