ArticlePLoS pathogens2018
EspL is essential for virulence and stabilizes EspE, EspF and EspH levels in Mycobacterium tuberculosis.
Article in PLoS pathogens, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Sec and Tat Mediated Secretion Safeguards Mycobacterium tuberculosis Membrane Homeostasis.Molecular & cellular proteomics : MCP · 2026Article
- Aromatic patch in whiB-like transcription factors facilitates primary sigma factor interaction in mycobacterium tuberculosis.Communications biology · 2026Article
- Assembly of theMicrobiology spectrum · 2025Article
- Gut microbiota and tuberculosis.iMeta · 2025Review
- The antagonistic transcription factors, EspM and EspN, regulate the ESX-1 secretion system inmBio · 2024Article
- Proteomics from compartment-specific APEX2 labeling in Mycobacterium tuberculosis reveals Type VII secretion substrates in the cell wall.Cell chemical biology · 2024Article
- Pathogenicity and virulence ofVirulence · 2023Review
- Functional Analysis of EspM, an ESX-1-Associated Transcription Factor in Mycobacterium marinum.Journal of bacteriology · 2022Article
- Proteo-genetic analysis reveals clear hierarchy of ESX-1 secretion inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Label-Free Comparative Proteomics of Differentially ExpressedPathogens (Basel, Switzerland) · 2021Article
- Modeling Tubercular ESX-1 Secretion Using Mycobacterium marinum.Microbiology and molecular biology reviews : MMBR · 2020Review
- Conserved ESX-1 Substrates EspE and EspF Are Virulence Factors That Regulate Gene Expression.Infection and immunity · 2020Article
- Polarly Localized EccEJournal of bacteriology · 2020Article
- Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Genetic Structure, Function, and Evolution of Capsule Biosynthesis Loci inFrontiers in microbiology · 2020Article
- Handling the Hurdles on the Way to Anti-tuberculosis Drug Development.Frontiers in chemistry · 2020Review
- Comparative Genomic and Transcriptomic Analyses ofFrontiers in cellular and infection microbiology · 2020Article
- Insight into the biology of Mycobacterium mucogenicum and Mycobacterium neoaurum clade members.Scientific reports · 2019Article
- ESX/Type VII Secretion Systems-An Important Way Out for Mycobacterial Proteins.Microbiology spectrum · 2019Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ESX-1, type VII, secretion system represents the major virulence determinant of Mycobacterium tuberculosis, one of the most successful intracellular pathogens. Here, by combining genetic and high-throughput approaches, we show that EspL, a protein of 115 amino acids, is essential for mediating ESX-1-dependent virulence and for stabilization of EspE, EspF and EspH protein levels. Indeed, an espL knock-out mutant was unable to replicate intracellularly, secrete ESX-1 substrates or stimulate innate cytokine production. Moreover, proteomic studies detected greatly reduced amounts of EspE, EspF and EspH in the espL mutant as compared to the wild type strain, suggesting a role for EspL as a chaperone. The latter conclusion was further supported by discovering that EspL interacts with EspD, which was previously demonstrated to stabilize the ESX-1 substrates and effector proteins, EspA and EspC. Loss of EspL also leads to downregulation in M. tuberculosis of WhiB6, a redox-sensitive transcriptional activator of ESX-1 genes. Overall, our data highlight the importance of a so-far overlooked, though conserved, component of the ESX-1 secretion system and begin to delineate the role played by EspE, EspF and EspH in virulence and host-pathogen interaction.
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