ArticlePlant biotechnology journal2019
Overexpression of SrUGT76G1 in Stevia alters major steviol glycosides composition towards improved quality.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 54 citations in OpenAlex.
- Development of In Vitro and In Planta Strategies for Efficient Production of High-Value Steviol Glycosides.Plant biotechnology journal · 2026Article
- Structural, evolutionary and functional insights into the NAC gene family in Stevia rebaudiana and identification of important genes regulating steviol glycosides biosynthesis.BMC plant biology · 2026Article
- Characterization ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Enhanced Production of Rebaudioside D and Rebaudioside M through V155T Substitution in the Glycosyltransferase UGT91D2 fromJournal of agricultural and food chemistry · 2025Article
- Improving organoleptic and antioxidant properties by inhibition of novel miRstv_7 to target key genes of steviol glycosides biosynthetic pathway in Stevia rebaudiana Bertoni.Plant molecular biology · 2024Article
- Genome-Wide Analysis of UGT Genes in Petunia and Identification ofPlants (Basel, Switzerland) · 2022Article
- DNA Free CRISPR/DCAS9 Based Transcriptional Activation System forPlants (Basel, Switzerland) · 2022Article
- Genome-wide identification, characterization, and expression analysis of UDP-glycosyltransferase genes associated with secondary metabolism in alfalfa (Frontiers in plant science · 2022Article
- Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (International journal of molecular sciences · 2021Article
- Metabolic engineering for the synthesis of steviol glycosides: current status and future prospects.Applied microbiology and biotechnology · 2021Review
- Synthesis and production of steviol glycosides: recent research trends and perspectives.Applied microbiology and biotechnology · 2021Review
- Co-immobilized recombinant glycosyltransferases efficiently convert rebaudioside A to M in cascade.RSC advances · 2021Article
- Steviol glycosides profile in Stevia rebaudiana Bertoni hairy roots cultured under oxidative stress-inducing conditions.Applied microbiology and biotechnology · 2020Article
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4 authors at 1 institution in 1 country.
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Abstract
Steviol glycosides (SGs) are extracted from Stevia leaves for use as a natural sweetener. Among SGs, stevioside is most abundant in leaf extracts followed by rebaudioside A (Reb A). However, Reb A is of particular interest because of its sweeter and more pleasant taste compared to stevioside. Therefore, the development of new Stevia varieties with a higher Reb A to stevioside ratio would be desirable for the production of higher quality natural sweeteners. Here, we generated transgenic Stevia plants overexpressing Stevia UDP-glycosyltransferase 76G1 (SrUGT76G1) that is known to convert stevioside to Reb A through 1,3-β-d-glucosylation in vitro. Interestingly, by overexpressing SrUGT76G1, the Reb A to stevioside ratio was drastically increased from 0.30 in wild-type (WT) plants up to 1.55 in transgenic lines without any significant changes in total SGs content. This was contributed by a concurrent increase in Reb A content and a decrease in stevioside content. Additionally, we were able to find an increase in the Reb C to dulcoside A ratio in transgenic lines. Using the glutathione S-transferase-tagged SrUGT76G1 recombinant protein for an in vitro glucosyltransferase assay, we further demonstrated that Reb C can be produced from the glucosylation of dulcoside A by SrUGT76G1. Transgenic Stevia plants having higher Reb A to stevioside ratio were visually indistinguishable from WT plants. Taken together, our results demonstrate that the overexpression of SrUGT76G1 in Stevia is an effective way to generate new Stevia varieties with higher proportion of the more preferred Reb A without compromising on plant development.
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