ArticleInternational journal of molecular medicine2019
Genipin protects against H2O2-induced oxidative damage in retinal pigment epithelial cells by promoting Nrf2 signaling.
Article in International journal of molecular medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 42 citations in OpenAlex.
- Comparative Action of Blue Food Colorants (Genipin, Patent Blue V, and Brilliant Blue FCF); Their Effect on Oxidative Stress in Human Plasma and Blood Platelets In Vitro.International journal of molecular sciences · 2026Article
- The In Vitro Antibacterial Effects of Genipin Against Pathogens Commonly Associated With Infected Ulcerative Keratitis: A Canine Preliminary Study.Investigative ophthalmology & visual science · 2026Article
- Ginkgolide B protects retinal pigment epithelial cells from HFrontiers in medicine · 2026Article
- Metabolic Symbiosis Between Cancer Cells and Endothelial Cells: A Key Driver of Tumor Angiogenesis.Advances in experimental medicine and biology · 2026Review
- Specific Assay Protocols for Porcine Single-Eye Retinal Pigment Epithelium Concerning Oxidative Stress and Inflammation.International journal of molecular sciences · 2025Article
- Artemisinin and Its Derivatives: Promising Therapeutic Agents for Age-Related Macular Degeneration.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Genipin-Simple but Significant Bioactive Iridoid for Therapeutical Application and Beyond: A Review.Life (Basel, Switzerland) · 2025Review
- Vitamin D alleviation of oxidative stress in human retinal pigment epithelial cells.International ophthalmology · 2024Article
- A Pilot Study to Evaluate Genipin inInternational journal of molecular sciences · 2023Article
- Inhibition of Mitochondrial Uncoupling Proteins Arrests Human Spermatozoa Motility without Compromising Viability.Antioxidants (Basel, Switzerland) · 2023Article
- Genipin protects against mitochondrial damage of the retinal pigment epithelium under hyperglycemia through theAnnals of translational medicine · 2022Article
- Novel Findings regarding the Bioactivity of the Natural Blue Pigment Genipin in Human Diseases.International journal of molecular sciences · 2022Review
- Genipin-Crosslinking Effects on Biomatrix Development for Cutaneous Wound Healing: A Concise Review.Frontiers in bioengineering and biotechnology · 2022Review
- Lycium barbarum polysaccharide protects ARPE‑19 cells against HMolecular medicine reports · 2021Article
- Medication Trends for Age-Related Macular Degeneration.International journal of molecular sciences · 2021Review
- Genipin in an Ex Vivo Corneal Model of Bacterial and Fungal Keratitis.Translational vision science & technology · 2021Article
- Anti-inflammatory activities ofExperimental and therapeutic medicine · 2021Article
- Smart Layer-by-Layer Polymeric Microreactors: pH-Triggered Drug Release and Attenuation of Cellular Oxidative Stress as Prospective Combination Therapy.ACS applied materials & interfaces · 2021Article
- Spermidine Attenuates Oxidative Stress-Induced Apoptosis via Blocking CaInternational journal of molecular sciences · 2021Article
- Cytoprotective Effects of Punicalagin on Hydrogen-Peroxide-Mediated Oxidative Stress and Mitochondrial Dysfunction in Retinal Pigment Epithelium Cells.Antioxidants (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxidative stress serves a vital function in the pathogenesis of age‑related macular degeneration (AMD); genipin (GP) possesses antioxidative properties. The present study aimed to investigate the effects of GP on retinal pigment epithelial (RPE) cells induced by H2O2 and the underlying mechanism. ARPE‑19 cells were subjected to H2O2 treatment to induce oxidative damage. Cell viability was determined via an MTT assay. Reactive oxygen species (ROS) levels and cell apoptosis were detected by flow cytometry. Nuclear factor‑erythroid 2‑related factor‑2 (Nrf2) signaling‑associated and the expression of apoptosis‑associated factors were measured using reverse transcription‑quantitative polymerase chain reaction assay and western blotting. The results revealed that 200 µM H2O2 and 30 µM GP were determined to be the optimal concentrations for subsequent experimentation. GP reversed the inhibitory effects of H2O2 by promoting cell viability, attenuating ROS accumulation and cell apoptosis, and increased the expression of Nrf2, heme oxygenase‑1 (HO‑1) and NAD(P)H: Quinine oxidoreductase 1 (NQO1); Nrf2 silencing inhibited HO‑1 and NQO1 expression. In addition, Nrf2 silencing enhanced the effects of H2O2 by promoting ROS production and cell apoptosis. Compared with H2O2, Nrf2 silencing further decreased the expression levels of B‑cell lymphoma‑2 (Bcl‑2), but increased that of Bcl‑2‑associated X protein and cleaved‑caspase‑3. The results of the present study revealed that Nrf2 silencing attenuated the protective effects of GP on H2O2‑induced injury in ARPE‑19 cells by promoting apoptosis and oxidation. Collectively, GP attenuated oxidative damage induced by H2O2 in ARPE‑19 cells. Furthermore, the molecular mechanism may be associated with the Nrf2 signaling pathway. The findings of the present study nay provide insight into a potential therapeutic agent for the treatment of AMD.
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