ArticlePlant methods2018
Reference genes identification for normalization of qPCR under multiple stresses in
Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Antibiotic resistance genes are more abundant in microplastic textile biofilms than natural cotton biofilms in freshwater.Applied and environmental microbiology · 2026Article
- Screening of qPCR Reference Genes in Quinoa Under Cold, Heat, and Drought Gradient Stress.Plants (Basel, Switzerland) · 2025Article
- Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis ofFood science & nutrition · 2025Article
- Validation of suitable reference microRNAs for qRT-PCR inFrontiers in plant science · 2025Article
- Identification and selection of reference genes for analysis of gene expression by quantitative real-time PCR in the euhalophyteCommunicative & integrative biology · 2024Article
- Selection of suitable reference lncRNAs for gene expression analysis inFrontiers in plant science · 2024Article
- Article
- Selection and Validation of Optimal RT-qPCR Reference Genes for the Normalization of Gene Expression under Different Experimental Conditions inPlants (Basel, Switzerland) · 2023Article
- Evaluating and Validating Sunflower Reference Genes for Q-PCR Studies Under High Temperature Condition.Iranian journal of biotechnology · 2023Article
- Article
- Validation of reference genes as an internal control for studying Avena sativa-Puccinia coronata interaction by RT-qPCR.Scientific reports · 2022Article
- Selection and Optimization of Reference Genes for MicroRNA Expression Normalization by qRT-PCR in Chinese Cedar (International journal of molecular sciences · 2021Article
- Validation of Reference Genes for Studying Different Abiotic Stresses in Oat (Plants (Basel, Switzerland) · 2021Article
- Selection and Verification of Appropriate Reference Genes for Expression Normalization inGenes · 2021Article
- Validation of suitable genes for normalization of diurnal gene expression studies in Chenopodium quinoa.PloS one · 2021Article
- Selection and Validation of Reference Genes for RT-qPCR Analysis inBioMed research international · 2021Article
- Reference gene validation in Eotetranychus sexmaculatus (Acari: Tetranychidae) feeding on mite-susceptible and mite-resistant rubber tree germplasms.Experimental & applied acarology · 2020Article
- Article
- Nanoparticles Affect the Expression Stability of Housekeeping Genes in Plant Cells.Nanotechnology, science and applications · 2020Article
- Visualization of spatial gene expression in plants by modified RNAscope fluorescent in situ hybridization.Plant methods · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundReal-time quantitative PCR has been widely used as the most reliable method to measure gene expression, due to its high accuracy and specificity. Wild barley (
resultsHere we report the first systematic and comprehensive analysis of reference genes for quantitative real-time PCR standardization in wild barley. We selected 11 genes, including
conclusionsOur main contribution was to identify reference genes with suitable and stable expression in wild barley under various stress conditions and in different tissues to provide a useful resource for future studies. The results demonstrate the importance of transcriptome data as a useful resource for the screening of candidate reference genes and highlight the need for specific reference genes for specific conditions. Furthermore, these findings will provide valuable information for wild barley and relative species for future research.
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