ArticleInternational journal of nanomedicine2018
A TEM-traceable physiologically functional gold nanoprobe that permeates non-endocytic cells.
Article in International journal of nanomedicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Plant-derived mitochondria mitigate aging-related neurodegeneration by reprogramming microglial mitochondrial energy metabolism.Translational neurodegeneration · 2026Article
- Expression, purification and application of a recombinant, membrane permeating version of the light chain of botulinum toxin B.Bioscience reports · 2024Article
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Authors and funding
3 authors.
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Abstract
backgroundNanoparticles' intracellular fate requires proper internalization. Most cells make use of a battery of internalization pathways, but some are practically sealed, as they lack the biochemical machinery for cellular intake. Non-endocytic cells, such as mammals' spermatozoa, challenge standard drug-delivery strategies. PURPOSE: In this article, we present a gold nanoprobe that permeates the external and internal membranes of human sperm.
methodsOur design makes use of a gold nanoparticle functionalized with a membrane-permeable cysteine-rich recombinant protein. The chimeric protein contains two units of physiologically active metallothioneins (MT) that also provide binding motifs to gold and a cell-penetrating-peptide sequence (CPP) that confers cell permeability to the nanoparticle.
resultsTransmission electron microscopy, indirect immunofluorescence, and functional assays show that the nanoprobe is readily internalized in sperm, without compromising cell integrity, while preserving MT's physiological activity. Our findings highlight the potential of CPP-functionalized nanogold for investigating the physiology of otherwise impermeable non-endocytic cells.
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