ArticleBMC veterinary research2018
Development of a lateral flow test for the rapid detection of Avibacterium paragallinarum in chickens suspected of having infectious coryza.
Article in BMC veterinary research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Translating conventional wisdom on chicken comb color into automated monitoring of disease-infected chicken using chromaticity-based machine learning models.Frontiers in veterinary science · 2023Article
- Immunoinformatic analysis of the whole proteome for vaccine design: An application toFrontiers in immunology · 2022Article
- A Highly Sensitive and Specific Probe-Based Real-Time PCR for the Detection ofFrontiers in veterinary science · 2021Article
- A Novel Lateral Flow Assay for Rapid and Sensitive Nucleic Acid Detection ofFrontiers in veterinary science · 2021Article
- A transient increase in MHC-IIVeterinary research · 2020Article
- Identification of Novel Candidate Epitopes on SARS-CoV-2 Proteins for South America: A Review of HLA Frequencies by Country.Frontiers in immunology · 2020Review
- Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region.Malaria journal · 2019Article
- Glycoprotein G (gG) production profile during infectious laryngotracheitis virus (ILTV) infection.PloS one · 2019Article
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Authors and funding
8 authors.
Funding
Abstract
backgroundInfectious coryza (IC) is an acute respiratory disease of growing chickens and layers caused by Avibacterium paragallinarum. The development of tools that allow rapid pathogen detection is necessary in order to avoid disease dissemination and economic losses in poultry. An Av. paragallinarum-specific Ma-4 epitope of the TonB-dependent transporter (TBDT) was selected using bioinformatic tools in order to immunize a BalbC mouse and to produce monoclonal antibodies to be used in a lateral flow test (LFT) developed for Av. paragallinarum detection in chicken nasal mucus samples.
resultsThe 1G7G8 monoclonal antibody was able to detect TBDT in Av. paragallinarum cultures (serogroups: A, B and C) by Western blot and indirect ELISA assay. Consequently, we developed a self-pairing prototype LFT. The limit of detection of the prototype LFT using Av. paragallinarum cultures was 1 × 10
conclusionsThe results indicate that the self-pairing prototype LFT is suitable for the detection of TBDT in Av. paragallinarum cultures as well as in field samples such as nasal mucus from Av. paragallinarum-infected chickens. Therefore, this prototype LFT could be considered a rapid and promising tool to be used in farm conditions for Av. paragallinarum diagnosis.
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