Evidence map›Paper›PMID 30567418›Full record

ArticleSensors (Basel, Switzerland)2018

Versatile Protein-A Coated Photoelectric Immunosensors with a Purple-Membrane Monolayer Transducer Fabricated by Affinity-Immobilization on a Graphene-Oxide Complexed Linker and by Shear Flow.

Hsueh-Hsia Wu, Xin-Quan Liao, Xin-Ying Wu, Cheng-De Lin, Kai-Ru Jheng, Hong-Ren Chen, Yong-Yi Wang, Hsiu-Mei Chen

Abstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hsueh-Hsia WuSchool of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan. wuhh@tmu.edu.tw.
Xin-Quan LiaoDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan. letter605@hotmail.com.
Xin-Ying WuDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan. ex8527@livemail.tw.
Cheng-De LinDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan. gn01657736@hotmail.com.
Kai-Ru JhengDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan. D9906104@mail.ntust.edu.tw.
Hong-Ren ChenDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan. kenneth991020@gmail.com.
Yong-Yi WangDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan. Bright4522@yahoo.com.tw.
Hsiu-Mei ChenDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan. hsiumei@mail.ntust.edu.tw.

Funding

Ministry of Science and Technology, Taiwan MOST 105-2221-E-011-132NTUST- TMU Joint Research Program NTUST-TMU-No. 107-04
6 · The paper itself

Abstract

Bacteriorhodopsin-embedded purple membranes (PM) have been demonstrated to be a sensitive photoelectric transducer for microbial detection. To efficiently prepare versatile BR-based immunosensors with protein A as antibody captures, a large, high-coverage, and uniformly oriented PM monolayer was fabricated on an electrode as an effective foundation for protein A conjugation through bis-NHS esters, by first affinity-coating biotinylated PM on an aminated surface using a complex of oxidized avidin and graphene oxide as the planar linker and then washing the coating with a shear flow. Three different polyclonal antibodies, each against

Indexed as

bacteriorhodopsingraphene oxidemonolayer fabricationphotoelectric immunosensorprotein Apurple membraneshear flow

Identifiers

PMID30567418
PMCPMC6308460

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.