ArticleAnnals of botany2019
The kinetics of ageing in dry-stored seeds: a comparison of viability loss and RNA degradation in unique legacy seed collections.
Article in Annals of botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- Seed Longevity in Legumes: Deeper Insights Into Mechanisms and Molecular Perspectives.Frontiers in plant science · 2022Pooled it
- Aging and longevity in decades-old genebanked seeds from U.S. endangered plant species: Assessments using survival and RNA integrity assays.American journal of botany · 2026Article
- Thermal behaviour of lipids in short-lived seeds of Australian rainforest species.Annals of botany · 2025Article
- Natural Ageing-Related Alterations of Biological Markers in Maize Seeds Under Ex-Situ Conservation.International journal of molecular sciences · 2025Article
- DNA Double Strand Break Repair Is Important for the Longevity of Primed Seeds.Plant, cell & environment · 2025Article
- Accumulating Heterozygous Deleterious Mutations in Conserved Soybean Germplasm over Successive Regenerations.Plants (Basel, Switzerland) · 2025Article
- Long-term preserved bean seeds exhibit high RNA integrity and high germination potential.Scientific reports · 2025Article
- Unraveling the Mechanistic Basis for Control of Seed Longevity.Plants (Basel, Switzerland) · 2025Review
- Assessing the RNA integrity in dry seeds collected from diverse endangered species native to the USA.Frontiers in plant science · 2025Article
- Screening of NIAS World Rice Core Collection for Seeds with Long Longevity as Useful Potential Breeding Materials Focusing on the Stability of Embryonic RNAs.Plants (Basel, Switzerland) · 2024Article
- Seed longevity and genome damage.Bioscience reports · 2024Review
- Viability and integrity ofConservation physiology · 2024Article
- Seed Longevity and Ageing: A Review on Physiological and Genetic Factors with an Emphasis on Hormonal Regulation.Plants (Basel, Switzerland) · 2023Review
- When Phased without Water: Biophysics of Cellular Desiccation, from Biomolecules to Condensates.Chemical reviews · 2023Review
- Seed Longevity-The Evolution of Knowledge and a Conceptual Framework.Plants (Basel, Switzerland) · 2023Review
- Comparative Seeds Storage Transcriptome Analysis ofInternational journal of molecular sciences · 2022Article
- Exploring Breakthroughs in Three Traits Belonging to Seed Life.Plants (Basel, Switzerland) · 2022Review
- A Critical Review of the Current Global Ex Situ Conservation System for Plant Agrobiodiversity. II. Strengths and Weaknesses of the Current System and Recommendations for Its Improvement.Plants (Basel, Switzerland) · 2021Review
- Correlation between seed longevity and RNA integrity in the embryos of rice seeds.Plant biotechnology (Tokyo, Japan) · 2021Article
- Analysis of Stored mRNA Degradation in Acceleratedly Aged Seeds of Wheat and Canola in Comparison to Arabidopsis.Plants (Basel, Switzerland) · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsDetermining seed longevity by identifying chemical changes that precede, and may be linked to, seed mortality, is an important but difficult task. The standard assessment, germination proportion, reveals seed longevity by showing that germination proportion declines, but cannot be used to predict when germination will be significantly compromised. Assessment of molecular integrity, such as RNA integrity, may be more informative about changes in seed health that precede viability loss, and has been shown to be useful in soybean.
methodsA collection of seeds stored at 5 °C and 35-50 % relative humidity for 1-30 years was used to test how germination proportion and RNA integrity are affected by storage time. Similarly, a collection of seeds stored at temperatures from -12 to +32 °C for 59 years was used to manipulate ageing rate. RNA integrity was calculated using total RNA extracted from one to five seeds per sample, analysed on an Agilent Bioanalyzer.
resultsDecreased RNA integrity was usually observed before viability loss. Correlation of RNA integrity with storage time or storage temperature was negative and significant for most species tested. Exceptions were watermelon, for which germination proportion and storage time were poorly correlated, and tomato, which showed electropherogram anomalies that affected RNA integrity number calculation. Temperature dependencies of ageing reactions were not significantly different across species or mode of detection. The overall correlation between germination proportion and RNA integrity, across all experiments, was positive and significant.
conclusionsChanges in RNA integrity when ageing is asymptomatic can be used to predict onset of viability decline. RNA integrity appears to be a metric of seed ageing that is broadly applicable across species. Time and molecular mobility of the substrate affect both the progress of seed ageing and loss of RNA integrity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.