ArticlePloS one2018
Size-dependent cytotoxicity of silver nanoparticles to Azotobacter vinelandii: Growth inhibition, cell injury, oxidative stress and internalization.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 171 citations in OpenAlex.
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- Gum Arabic: A Commodity with Versatile Formulations and Applications.Nanomaterials (Basel, Switzerland) · 2025Review
- Nanoparticles and root traits: mineral nutrition, stress tolerance and interaction with rhizosphere microbiota.Planta · 2024Review
- Glycyrrhizic Acid Nanoparticles Subside the Activity of Methicillin-ResistantPharmaceuticals (Basel, Switzerland) · 2024Article
- The Effects of Silver Nanoparticles (AgNPs) on Thermophilic Bacteria: Antibacterial, Morphological, Physiological and Biochemical Investigations.Microorganisms · 2024Article
- Article
- The Green Synthesis of Silver Nanoparticles fromPathogens (Basel, Switzerland) · 2023Article
- Rising Influence of Nanotechnology in Addressing Oxidative Stress-Related Liver Disorders.Antioxidants (Basel, Switzerland) · 2023Review
- The Role of Crosslinker Content of Positively Charged NIPAM Nanogels on the In Vivo Toxicity in Zebrafish.Pharmaceutics · 2023Article
- Inhibition Mechanism of Methicillin-ResistantACS omega · 2023Article
- Hybrid Nanosystems of Antibiotics with Metal Nanoparticles-Novel Antibacterial Agents.Molecules (Basel, Switzerland) · 2023Review
- Bio-functionalized nickel-silica nanoparticles suppress bacterial leaf blight disease in rice (Frontiers in plant science · 2023Article
- Bioinspired Green Synthesis of Silver Nanoparticles Using Three Plant Extracts and Their Antibacterial Activity against Rice Bacterial Leaf Blight PathogenPlants (Basel, Switzerland) · 2022Article
- Evaluation of TiOIndian journal of microbiology · 2022Review
- New formula of the green synthesised Au@Ag core@shell nanoparticles using propolis extract presented high antibacterial and anticancer activity.AMB Express · 2022Article
- Biological Synthesis of Silver Nanoparticles and Prospects in Plant Disease Management.Molecules (Basel, Switzerland) · 2022Review
- Nanoparticles of Bioactive Metals/Metal Oxides and Their Nanocomposites with Antibacterial Drugs for Biomedical Applications.Materials (Basel, Switzerland) · 2022Review
- Evaluation of the Effects of Ag, Cu, ZnO and TiOInternational journal of molecular sciences · 2022Article
- Comparative study of antifungal activity of two preparations of green silver nanoparticles fromSaudi journal of biological sciences · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The influence of nanomaterials on the ecological environment is becoming an increasingly hot research field, and many researchers are exploring the mechanisms of nanomaterial toxicity on microorganisms. Herein, we studied the effect of two different sizes of nanosilver (10 nm and 50 nm) on the soil nitrogen fixation by the model bacteria Azotobacter vinelandii. Smaller size AgNPs correlated with higher toxicity, which was evident from reduced cell numbers. Flow cytometry analysis further confirmed this finding, which was carried out with the same concentration of 10 mg/L for 12 h, the apoptotic rates were20.23% and 3.14% for 10 nm and 50 nm AgNPs, respectively. Structural damage to cells were obvious under scanning electron microscopy. Nitrogenase activity and gene expression assays revealed that AgNPs could inhibit the nitrogen fixation of A. vinelandii. The presence of AgNPs caused intracellular reactive oxygen species (ROS) production and electron spin resonance further demonstrated that AgNPs generated hydroxyl radicals, and that AgNPs could cause oxidative damage to bacteria. A combination of Ag content distribution assays and transmission electron microscopy indicated that AgNPs were internalized in A. vinelandii cells. Overall, this study suggested that the toxicity of AgNPs was size and concentration dependent, and the mechanism of antibacterial effects was determined to involve damage to cell membranes and production of reactive oxygen species leading to enzyme inactivation, gene down-regulation and death by apoptosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.