ArticleBiochemistry2019
Template-Directed Catalysis of a Multistep Reaction Pathway for Nonenzymatic RNA Primer Extension.
Article in Biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- MgJournal of the American Chemical Society · 2026Article
- Article
- Overcoming nucleotide bias in the nonenzymatic copying of RNA templates.Nucleic acids research · 2024Article
- Simulations predict preferred MgBiophysical journal · 2024Article
- Catalytic Metal Ion-Substrate Coordination during Nonenzymatic RNA Primer Extension.Journal of the American Chemical Society · 2024Article
- Unusual Base Pair between Two 2-Thiouridines and Its Implication for Nonenzymatic RNA Copying.Journal of the American Chemical Society · 2024Article
- Towards a prebiotic chemoton - nucleotide precursor synthesis driven by the autocatalytic formose reaction.Chemical science · 2023Article
- Kinetic explanations for the sequence biases observed in the nonenzymatic copying of RNA templates.Nucleic acids research · 2022Article
- Primordial mimicry induces morphological change in Escherichia coli.Communications biology · 2022Article
- Sequencing the origins of life.BBA advances · 2022Review
- When Is a Reaction Network a Metabolism? Criteria for Simple Metabolisms That Support Growth and Division of Protocells.Life (Basel, Switzerland) · 2021Article
- Competition between bridged dinucleotides and activated mononucleotides determines the error frequency of nonenzymatic RNA primer extension.Nucleic acids research · 2021Article
- The virtual circular genome model for primordial RNA replication.RNA (New York, N.Y.) · 2021Article
- Review
- Potentially Prebiotic Activation Chemistry Compatible with Nonenzymatic RNA Copying.Journal of the American Chemical Society · 2020Article
- In vitro selection of ribozyme ligases that use prebiotically plausible 2-aminoimidazole-activated substrates.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Before the advent of polymerase enzymes, the copying of genetic material during the origin of life may have involved the nonenzymatic polymerization of RNA monomers that are more reactive than the biological nucleoside triphosphates. Activated RNA monomers such as nucleotide 5'-phosphoro-2-aminoimidazolides spontaneously form an imidazolium-bridged dinucleotide intermediate that undergoes rapid nonenzymatic template-directed primer extension. However, it is unknown whether the intermediate can form on the template or only in solution and whether the intermediate is prone to hydrolysis when bound to the template or reacts preferentially with the primer. Here we show that an activated monomer can first bind the template and then form an imidazolium-bridged intermediate by reacting with a 2-aminoimidazole-activated downstream oligonucleotide. We have also characterized the partition of the template-bound intermediate between hydrolysis and primer extension. In the presence of the catalytic metal ion Mg
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