ArticleMethods in molecular biology (Clifton, N.J.)2019
Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence.
Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Regulation of Hippo signaling by Atrophin in the developing Drosophila wing.Development (Cambridge, England) · 2026Article
- Jub-induced phosphorylation of Warts inhibits its activity and recruitment into biomolecular condensates.Cell reports · 2025Article
- Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling.Development (Cambridge, England) · 2024Article
- The Interaction of Mechanics and the Hippo Pathway inCancers · 2023Article
- Peripodial adherens junctions regulate Ajuba-Yorkie signaling to preserve fly eye morphology.Biology open · 2023Article
- Near-infrared nuclear markers formicroPublication biology · 2022Article
- Analysis of the Drosophila Ajuba LIM protein defines functions for distinct LIM domains.PloS one · 2022Article
- Organization and function of tension-dependent complexes at adherens junctions.Journal of cell science · 2019Article
- Recruitment of Jub by α-catenin promotes Yki activity andJournal of cell science · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Visualization of in vivo protein levels and localization is essential to analysis and elucidation of Hippo signaling mechanisms and its roles in diverse tissues. This is best done by imaging proteins using fluorescent labels. Fluorescent labeling of a protein can be achieved by direct conjugation to an intrinsically fluorescent protein, like GFP, or by use of antibodies conjugated to fluorescent dyes. Immunofluorescence imaging in Drosophila typically begins with dissection and fixation of a sample tissue, followed by a series of washes and incubations with primary antibodies, directed against proteins of interest, and dye-labeled secondary antibodies, directed against the primary antibodies. This may be followed by fluorescent dyes that label cellular components, such as DNA-labeling dyes to mark nuclei. After staining and washing is completed, samples are placed in a mounting media, transferred to a microscope slide, and imaged on a confocal microscope.
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Registered trials
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