Evidence map›Paper›PMID 30564919›Full record

ArticleMedical microbiology and immunology2019

Increased SAMHD1 transcript expression correlates with interferon-related genes in HIV-1-infected patients.

Maura Statzu, Letizia Santinelli, Agnese Viscido, Claudia Pinacchio, Giancarlo Ceccarelli, Claudia Rotondo, Giuseppe Corano Scheri, Ivano Mezzaroma, Ombretta Turriziani, Guido Antonelli and 2 more

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Article in Medical microbiology and immunology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Maura StatzuLaboratory of Virology, Department of Molecular Medicine, Affiliated to Istituto Pasteur Italia, Cenci Bolognetti Foundation, Sapienza University of Rome, Rome, Italy.
Letizia SantinelliLaboratory of Virology, Department of Molecular Medicine, Affiliated to Istituto Pasteur Italia, Cenci Bolognetti Foundation, Sapienza University of Rome, Rome, Italy.
Agnese ViscidoLaboratory of Virology, Department of Molecular Medicine, Affiliated to Istituto Pasteur Italia, Cenci Bolognetti Foundation, Sapienza University of Rome, Rome, Italy.
Claudia PinacchioDepartment of Public Health and Infectious Diseases, Sapienza University of Rome, Policlinico Umberto I, Viale del Policlinico 155, 00161, Rome, Italy.
Giancarlo CeccarelliDepartment of Public Health and Infectious Diseases, Sapienza University of Rome, Policlinico Umberto I, Viale del Policlinico 155, 00161, Rome, Italy.
Claudia RotondoLaboratory of Virology, Department of Molecular Medicine, Affiliated to Istituto Pasteur Italia, Cenci Bolognetti Foundation, Sapienza University of Rome, Rome, Italy.
Giuseppe Corano ScheriDepartment of Public Health and Infectious Diseases, Sapienza University of Rome, Policlinico Umberto I, Viale del Policlinico 155, 00161, Rome, Italy.
Ivano MezzaromaDepartment of Translational and Precision Medicine, Sapienza University of Rome, Rome, Italy.
Ombretta TurrizianiLaboratory of Virology, Department of Molecular Medicine, Affiliated to Istituto Pasteur Italia, Cenci Bolognetti Foundation, Sapienza University of Rome, Rome, Italy.
Guido AntonelliLaboratory of Virology, Department of Molecular Medicine, Affiliated to Istituto Pasteur Italia, Cenci Bolognetti Foundation, Sapienza University of Rome, Rome, Italy.
Gabriella d'EttorreDepartment of Public Health and Infectious Diseases, Sapienza University of Rome, Policlinico Umberto I, Viale del Policlinico 155, 00161, Rome, Italy.
Carolina ScagnolariLaboratory of Virology, Department of Molecular Medicine, Affiliated to Istituto Pasteur Italia, Cenci Bolognetti Foundation, Sapienza University of Rome, Rome, Italy. carolina.scagnolari@uniroma1.it.
Sapienza University of Rome · ITIstituto Pasteur · ITPoliclinico Umberto I · IT

Funding

Sapienza Università di Roma RM11715C586062AF
6 · The paper itself

Abstract

purposeTo investigate the contribution of SAMHD1 to HIV-1 infection in vivo and its relationship with IFN response, the expression of SAMHD1 and IFN-related pathways was evaluated in HIV-1-infected patients.

methodsPeripheral blood mononuclear cells (PBMC) from 388 HIV-1-infected patients, both therapy naïve (n = 92) and long-term HAART treated (n = 296), and from 100 gender and age-matched healthy individuals were examined. CD4+ T cells, CD14+ monocytes and gut biopsies were also analyzed in HIV-1-infected subjects on suppressive antiretroviral therapy. Gene expression levels of SAMDH1, ISGs (MxA, MxB, HERC5, IRF7) and IRF3 were evaluated by real-time RT-PCR assays.

resultsSAMHD1 levels in HIV-1-positive patients were significantly increased compared to those in healthy donors. SAMHD1 expression was enhanced in treated patients compared to naïve patients (p < 0.0001) and healthy donors (p = 0.0038). Virologically suppressed treated patients exhibited higher SAMHD1 levels than healthy donors (p = 0.0008), viraemic patients (p = 0.0001) and naïve patients (p < 0.0001). SAMHD1 levels were also increased in CD4+ T cells compared to those in CD14+ monocytes and in PBMC compared to those of GALT. Moreover, SAMHD1 was expressed more strongly than ISGs in HIV-1-infected patients and positive correlations were found between SAMHD1, ISGs and IRF3 levels.

conclusionsSAMHD1 is more strongly expressed than the classical IFN-related genes, increased during antiretroviral therapy and correlated with ISGs and IRF3 in HIV-1-infected patients.

Indexed as

Gene ExpressionAdultAgedFemaleGene Expression ProfilingHIV InfectionsHumansImmunologic FactorsLeukocytes, MononuclearMaleMiddle AgedSAM Domain and HD Domain-Containing Protein 1Young AdultImmunologic FactorsSAM Domain and HD Domain-Containing Protein 1SAMHD1 protein, humanHIV-1IFNIRF3ISGSAMHD1

Identifiers

PMID30564919
OpenAlexW2904372983

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.