Evidence map›Paper›PMID 30564876›Full record

ArticleMedical molecular morphology2019

Knockdown of long non-coding RNA PCAT1 in glioma stem cells promotes radiation sensitivity.

Penghai Zhang, Yang Liu, Changyu Fu, Ce Wang, Xingbang Duan, Wenting Zou, Tianshu Zhao

Abstract read
PubMed Publisher
In one paragraph

Article in Medical molecular morphology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
1.0field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Penghai ZhangDepartment of Neurosurgery, Heilongjiang Provincial Hospital, No. 82 Zhongshan Road, Xiangfang District, Harbin, 150030, Heilongjiang, China.
Yang LiuDepartment of Neurosurgery, Heilongjiang Provincial Hospital, No. 82 Zhongshan Road, Xiangfang District, Harbin, 150030, Heilongjiang, China.
Changyu FuDepartment of Neurosurgery, The Fourth Affiliated Hospital, Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin, 150001, Heilongjiang, China.
Ce WangDepartment of Neurosurgery, The Fourth Affiliated Hospital, Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin, 150001, Heilongjiang, China.
Xingbang DuanDepartment of Neurosurgery, The Fourth Affiliated Hospital, Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin, 150001, Heilongjiang, China.
Wenting ZouDepartment of Neurosurgery, Heilongjiang Provincial Hospital, No. 82 Zhongshan Road, Xiangfang District, Harbin, 150030, Heilongjiang, China. zlxia01@126.com.
Tianshu ZhaoDepartment of Neurosurgery, The Fourth Affiliated Hospital, Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin, 150001, Heilongjiang, China. cyy_605@163.com.ORCID http://orcid.org/0000-0002-3915-546X
Harbin Medical University · CNHeilongjiang Provincial Hospital · CN

Funding

Harbin Science and Technology Talent Innovation Fund Project 2016RAQXJ173Heilongjiang Province Health and Family Planning Commission Research 2016-129
6 · The paper itself

Abstract

This study aimed to investigate the function of glioma stem cells (GSCs) and the role of PCAT1. This study dissociated the differences between GSCs and glioma cells in terms of apoptosis rate and γH2AX positive cells levels after radiation. Microarray was carried out to detect that expressed PCAT1, and it was testified by RT-qPCR. After transfection, GSCs were used to investigate the influence of PCAT1 on radiation sensitivity. Sphere-formation capability was first examined. Cell apoptosis rate after radiation of 0 Gy or 6 Gy was analyzed by flow cytometry, and the level of γH2AX positive cells after 6 Gy radiation were compared. CCK8 assay was used to investigate the cell proliferation and RT-qPCR was used to examine miR-129-5p and HMGB1 expression. GSCs exhibited great capability in sphere formation and lower expression in apoptosis and γH2AX positive cells rates after 6 Gy radiation. PCAT1 had higher expression in GSCs. PCAT1 knockdown restrained the sphere-formation ability, increased the apoptosis rate and DNA damage under the treatment of radiation. Moreover, knockdown of PCAT1 inhibited the cell proliferation. In addition, silencing PCAT1 could increase the expression of miR-129-5p and decrease the expression of HMGB1. PCAT1 was overexpressed in GSCs and played a facilitating role in radiation resistance.

Indexed as

ApoptosisBrain NeoplasmsCell Line, TumorCell ProliferationGene Expression ProfilingGene Expression Regulation, NeoplasticGliomaHumansNeoplastic Stem CellsRadiation ToleranceRNA, Long NoncodingSpheroids, CellularRNA, Long NoncodingGliomaGSCPCAT1RadiationγH2AX

Identifiers

PMID30564876
OpenAlexW2905017500

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.