ArticleLight, science & applications2018
Full-field interferometric imaging of propagating action potentials.
Article in Light, science & applications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The trial behind it
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Who cites it
31 citing papers in PubMed, 67 citations in OpenAlex.
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- The wave nature of the action potential.Frontiers in cellular neuroscience · 2025Article
- High-resolution transcranial optical imaging of in vivo neural activity.Scientific reports · 2024Article
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- Neurocomputational mechanisms underlying perception and sentience in the neocortex.Frontiers in computational neuroscience · 2024Review
- Asymmetric metasurface photodetectors for single-shot quantitative phase imaging.Nanophotonics (Berlin, Germany) · 2023Article
- Phase-sensitive detection of anomalous diffusion dynamics in the neuronal membrane induced by ion channel gating.Physics in medicine and biology · 2023Article
- Acousto-holographic reconstruction of whole-cell stiffness maps.Nature communications · 2022Article
- Ultrafast and hypersensitive phase imaging of propagating internodal current flows in myelinated axons and electromagnetic pulses in dielectrics.Nature communications · 2022Article
- On Some Current Challenges in High-Resolution Optical Bioimaging.ACS photonics · 2022Review
- Ultra-parallel label-free optophysiology of neural activity.iScience · 2022Article
- Optical Electrophysiology: Toward the Goal of Label-Free Voltage Imaging.Journal of the American Chemical Society · 2021Article
- Spatio-temporal performance in an incoherent holography lattice light-sheet microscope (IHLLS).Optics express · 2021Article
- Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry.Nature communications · 2021Article
- Does the Brain Function as a Quantum Phase Computer Using Phase Ternary Computation?Frontiers in physiology · 2021Review
- Detection of cellular micromotion by advanced signal processing.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Currently, cellular action potentials are detected using either electrical recordings or exogenous fluorescent probes that sense the calcium concentration or transmembrane voltage. Ca imaging has a low temporal resolution, while voltage indicators are vulnerable to phototoxicity, photobleaching, and heating. Here, we report full-field interferometric imaging of individual action potentials by detecting movement across the entire cell membrane. Using spike-triggered averaging of movies synchronized with electrical recordings, we demonstrate deformations up to 3 nm (0.9 mrad) during the action potential in spiking HEK-293 cells, with a rise time of 4 ms. The time course of the optically recorded spikes matches the electrical waveforms. Since the shot noise limit of the camera (~2 mrad/pix) precludes detection of the action potential in a single frame, for all-optical spike detection, images are acquired at 50 kHz, and 50 frames are binned into 1 ms steps to achieve a sensitivity of 0.3 mrad in a single pixel. Using a self-reinforcing sensitivity enhancement algorithm based on iteratively expanding the region of interest for spatial averaging, individual spikes can be detected by matching the previously extracted template of the action potential with the optical recording. This allows all-optical full-field imaging of the propagating action potentials without exogeneous labels or electrodes.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.