ArticleFrontiers in microbiology2018
Minimal Influence of Extracellular DNA on Molecular Surveys of Marine Sedimentary Communities.
Article in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Improving biocide evaluation using propidium monoazide (PMA) viability staining technique.Scientific reports · 2025Article
- Bacterial community response to novel and repeated disturbances.Environmental microbiology reports · 2024Article
- DNA and RNA Stability of Marine Microalgae in Cold-Stored Sediments and Its Implications in Metabarcoding Analyses.International journal of molecular sciences · 2024Article
- Article
- Permafrost microbial communities and functional genes are structured by latitudinal and soil geochemical gradients.The ISME journal · 2023Article
- Why eDNA fractions need consideration in biomonitoring.Molecular ecology resources · 2022Review
- Review
- A Critical Assessment of the Congruency between Environmental DNA and Palaeoecology for the Biodiversity Monitoring and Palaeoenvironmental Reconstruction.International journal of environmental research and public health · 2022Review
- Long-term preservation of biomolecules in lake sediments: potential importance of physical shielding by recalcitrant cell walls.PNAS nexus · 2022Article
- Introducing Candidatus Bathyanammoxibiaceae, a family of bacteria with the anammox potential present in both marine and terrestrial environments.ISME communications · 2022Article
- Extracellular DNA in Environmental Samples: Occurrence, Extraction, Quantification, and Impact on Microbial Biodiversity Assessment.Applied and environmental microbiology · 2022Article
- Cryptic metabolisms in anoxic subseafloor sediment.Environmental microbiology reports · 2021Article
- Genomic insights into diverse bacterial taxa that degrade extracellular DNA in marine sediments.Nature microbiology · 2021Article
- Carbonate-hosted microbial communities are prolific and pervasive methane oxidizers at geologically diverse marine methane seep sites.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Microbial Community Response to Polysaccharide Amendment in Anoxic Hydrothermal Sediments of the Guaymas Basin.Frontiers in microbiology · 2021Article
- The masking effect of extracellular DNA and robustness of intracellular DNA in anaerobic digester NGS studies: A discriminatory study of the total DNA pool.Molecular ecology · 2021Article
- Article
- Article
- Microbial Community Structure in Arctic Lake Sediments Reflect Variations in Holocene Climate Conditions.Frontiers in microbiology · 2020Article
- Changes in the Active, Dead, and Dormant Microbial Community Structure across a Pleistocene Permafrost Chronosequence.Applied and environmental microbiology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular DNA has been reported to comprise a large fraction of total DNA in near-seafloor sediment. However, the potential effect of extracellular DNA, arising from dead or moribund cells, on sequencing surveys is a critical concern that has largely not been addressed for marine sedimentary habitats. To address this concern, we interrogated freshly collected Arctic and Pacific sediment for extracellular 16S rRNA genes using the photoactive DNA-binding dye Propidium Monoazide. Significant differences between relative abundances of total (intracellular + extracellular) Bacterial 16S rRNA genes and relative abundances of intracellular Bacterial 16S rRNA genes are only detected in three of twelve shallow [10 cm below seafloor (cmbsf)] samples. Relative abundances of total Bacterial 16S rRNA genes are statistically indistinguishable from relative abundances of intracellular Bacterial 16S rRNA genes in all interrogated samples from depths greater than 10 cmbsf. 16S rRNA gene sequencing shows that even where significantly higher abundances of extracellular genes are detected, they have little or no influence on prokaryote community composition. Taxon-level analyses suggest that extracellular DNA, arising from
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.