Evidence map›Paper›PMID 30563457›Full record

ArticleBMC plant biology2018

The effects of mepiquat chloride on the lateral root initiation of cotton seedlings are associated with auxin and auxin-conjugate homeostasis.

Xiaojiao Chen, Man Zhang, Mian Wang, Guiyu Tan, Mingcai Zhang, Yu Xia Hou, Baomin Wang, Zhaohu Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.4field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Xiaojiao ChenCollege of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
Man ZhangCollege of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
Mian WangCollege of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
Guiyu TanCollege of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
Mingcai ZhangCollege of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
Yu Xia HouCollege of Science, China Agricultural University, Beijing, 100193, China.
Baomin WangCollege of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. wbaomin@263.net.ORCID http://orcid.org/0000-0002-4235-198X
Zhaohu LiCollege of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
China Agricultural University · CN

Funding

National Natural Science Foundation of China Grant No. 31471434
6 · The paper itself

Abstract

backgroundMepiquat chloride (MC) is a plant growth regulator widely used in cotton (Gossypium hirsutum L.) production to suppress excessive vegetative growth, increase root growth and avoid yield losses. To increase root growth, cotton seeds were treated with MC to increase the number of lateral root (LRs) and improve drought resistance. An increased indole-3-acetic acid (IAA) pool appeared to correlate with LR growth, and the principal source of IAA in germinating seeds is IAA conjugates. Here, the role of IAA homeostasis and signaling was investigated in cotton seedlings treated with MC.

resultsIn the present research, MC significantly increased endogenous IAA levels in the roots, which promoted lateral root initiation (LRI) by upregulating GhARF7/19 and GhLBD18s and subsequently increasing LR quantity and elongation. The levels of IAA-amide conjugates significantly decreased in MC-treated seedlings compared with untreated control seedlings. Sixteen members of the cotton IAA amidohydrolase (IAH) gene family were identified, of which GhIAR3a, GhIAR3b, GhILR1, GhILL3 and GhILL6 were expressed during cotton seed germination. Compared with those in untreated control seedlings, the expression levels of GhIAR3a, GhIAR3b, GhILR1 and GhILL6 in the MC-treated seedlings were markedly elevated. The GhIAR3a/b and GhILR1 genes were cloned and expressed in Escherichia coli; these recombinant proteins exhibited hydrolytic activity that could cleave IAA-phenyalanine (Phe), IAA-methionine (Met), IAA-glycine (Gly) and IAA-leucine (Leu) in vitro, while only GhIAR3a hydrolyzed IAA-alanine (Ala) efficiently. The content of GhIAR3a, as detected via an established sandwich enzyme-linked immunosorbent assay (ELISA), increased in the MC-treated seedlings compared with the untreated control seedlings. In addition, the Arabidopsis iar3 mutant was less responsive to MC-induced LR growth than was wild type.

conclusionsThese findings suggested that MC application could mediate IAA homeostasis via increased IAA levels from IAA-amide conjugate hydrolysis by accelerating IAH gene expression, which might promote LRI and increase the LR quantity and elongation.

Indexed as

AmidohydrolasesAnimalsAntibodies, MonoclonalArabidopsisArabidopsis ProteinsCloning, MolecularEnzyme-Linked Immunosorbent AssayGene Expression Regulation, PlantGossypiumHomeostasisIndoleacetic AcidsMiceMutationPiperidinesPlant Growth RegulatorsPlant ProteinsAmidohydrolasesAntibodies, MonoclonalArabidopsis ProteinsIAR3 protein, Arabidopsisindoleacetic acidIndoleacetic AcidsmepiquatPiperidinesPlant Growth RegulatorsPlant ProteinsAmidohydrolaseGossypium hirsutum L.IAAIAA-amide conjugatesLateral root initiationMepiquat chloride

Identifiers

PMID30563457
PMCPMC6299555
OpenAlexW2905485989

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.