Evidence map›Paper›PMID 30563214›Full record

ArticleCells2018

Effect of a Collagen-Based Compound on Morpho-Functional Properties of Cultured Human Tenocytes.

Filippo Randelli, Alessandra Menon, Alessio Giai Via, Manuel Giovanni Mazzoleni, Fabio Sciancalepore, Marco Brioschi, Nicoletta Gagliano

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 40 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Filippo RandelliCentro di Chirurgia dell'Anca e Traumatologia, I.R.C.C.S Policlinico San Donato, 20097 San Donato Milanese, Italy. filippo.randelli@fastwebnet.it.ORCID 0000-0002-7643-0512
Alessandra MenonAzienda Socio Sanitaria Territoriale Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, 1^ Clinica Ortopedica, 20122 Milan, Italy. ale.menon@me.com.
Alessio Giai ViaCentro di Chirurgia dell'Anca e Traumatologia, I.R.C.C.S Policlinico San Donato, 20097 San Donato Milanese, Italy. alessiogiaivia@hotmail.it.
Manuel Giovanni MazzoleniCentro di Chirurgia dell'Anca e Traumatologia, I.R.C.C.S Policlinico San Donato, 20097 San Donato Milanese, Italy. manuelmazzoleni4@gmail.com.ORCID 0000-0002-8802-7122
Fabio SciancaleporeAzienda Socio Sanitaria Territoriale Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, 1^ Clinica Ortopedica, 20122 Milan, Italy. fabio.sciancalepore@unimi.it.
Marco BrioschiCentro di Chirurgia dell'Anca e Traumatologia, I.R.C.C.S Policlinico San Donato, 20097 San Donato Milanese, Italy. marco.brioschi@unimi.it.ORCID 0000-0002-0217-4595
Nicoletta GaglianoDepartment of Biomedical Sciences for Health, Università degli Studi di Milano, 20133 Milan, Italy. nicoletta.gagliano@unimi.it.ORCID 0000-0002-3393-3144
IRCCS Policlinico San Donato · ITIstituto Ortopedico Gaetano Pini · ITUniversity of Milan · IT

Funding

Guna -
6 · The paper itself

Abstract

backgroundGreater Trochanter Pain Syndrome (GTPS) is the main reason for recalcitrant lateral hip pain. Gluteus medius and minimus tendinopathy plays a key role in this setting. An injectable medical compound containing collagen type I (MD-Tissue, Guna) has been produced with the aim to counteract the physiological and pathological degeneration of tendons. In this study we aimed at characterizing the effect of this medical compound on cultured human gluteal tenocytes, focusing on the collagen turnover pathways, in order to understand how this medical compound could influence tendon biology and healing.

methodsTenocytes were obtained from gluteal tendon fragments collected in eight patients without any gluteal tendon pathology undergoing total hip replacement through an anterior approach. Cell proliferation and migration were investigated by growth curves and wound healing assay, respectively. The expression of genes and proteins involved in collagen turnover were analysed by real-time PCR, Slot blot and SDS-zymography.

resultsOur data show that tenocytes cultured on MD-Tissue, compared to controls, have increased proliferation rate and migration potential. MD-Tissue induced collagen type I (COL-I) secretion and mRNA levels of tissue inhibitor of matrix metalloproteinases (MMP)-1 (TIMP-1). Meanwhile, lysyl hydroxylase 2b and matrix metalloproteinases (MMP)-1 and -2, involved, respectively, in collagen maturation and degradation, were not affected.

conclusionsConsidered as a whole, our results suggest that MD-Tissue could induce in tenocytes an anabolic phenotype by stimulating tenocyte proliferation and migration and COL-I synthesis, maturation, and secretion, thus favouring tendon repair. In particular, based on its effect on gluteal tenocytes, MD-Tissue could be effective in the discouraging treatment of GTPS. From now a rigorous clinical investigation is desirable to understand the real clinical potentials of this compound.

Indexed as

collagen turnovercytoskeletonfocal adhesionGreater Trochanter Pain Syndromematrix metalloproteinasesTendinopathytendon

Identifiers

PMID30563214
PMCPMC6316559
OpenAlexW2902525931

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.