ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2019
Factors Affecting Ultrastructural Quality in the Prefrontal Cortex of the Postmortem Human Brain.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed.
- Effects of postmortem interval on myelin integrity in rhesus macaque brain tissue.Brain pathology (Zurich, Switzerland) · 2026Article
- Organ donation and brain preservation after assisted dying: case report and considerations for a potential pathway.BMC medical ethics · 2026Article
- Exploring the human brain: spatial transcriptomics challenges and approaches in post-mortem analysis.Brain : a journal of neurology · 2026Review
- Imaging myelin degradation in ex vivo prefrontal cortex tissue blocks in Alzheimer's disease and chronic traumatic encephalopathy.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2025Article
- Volume electron microscopy reveals 3D synaptic nanoarchitecture in postmortem human prefrontal cortex.iScience · 2025Article
- Modeling Parkinson's disease pathology in human dopaminergic neurons by sequential exposure to α-synuclein fibrils and proinflammatory cytokines.Nature neuroscience · 2024Article
- Biostasis: A Roadmap for Research in Preservation and Potential Revival of Humans.Brain sciences · 2024Review
- Comparing stem cells, transdifferentiation and brain organoids as tools for psychiatric research.Translational psychiatry · 2024Review
- Immersion Fixation and Staining of Multicubic Millimeter Volumes for Electron Microscopy-Based Connectomics of Human Brain Biopsies.Biological psychiatry · 2023Article
- Interhemispheric differences of pyramidal cells in the primary motor cortices of schizophrenia patients investigated postmortem.Cerebral cortex (New York, N.Y. : 1991) · 2023Article
- Cross-Platform Synaptic Network Analysis of Human Entorhinal Cortex Identifies TWF2 as a Modulator of Dendritic Spine Length.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Postmortem changes in brain cell structure: a review.Free neuropathology · 2023Article
- Microglia in Human Postmortem Brain Samples: Quantitative Ultrastructural Analysis of Scanning Electron Microscopy Images.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Neurexin and neuroligins jointly regulate synaptic degeneration at theFrontiers in cellular neuroscience · 2023Article
- Reconstruction of ovine axonal cytoarchitecture enables more accurate models of brain biomechanics.Communications biology · 2022Article
- Epigenome-wide association study of alcohol use disorder in five brain regions.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2022Article
- Three-Dimensional Synaptic Organization of Layer III of the Human Temporal Neocortex.Cerebral cortex (New York, N.Y. : 1991) · 2021Article
- Preservation of global synaptic excitatory to inhibitory ratio during long postmortem intervals.Scientific reports · 2020Article
- Optimization of ultrastructural preservation of human brain for transmission electron microscopy after long post-mortem intervals.Acta neuropathologica communications · 2019Article
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Abstract
Electron microscopy (EM) studies of the postmortem human brain provide a level of resolution essential for understanding brain function in both normal and disease states. However, processes associated with death can impair the cellular and organelle ultrastructural preservation required for quantitative EM studies. Although postmortem interval (PMI), the time between death and preservation of tissue, is thought to be the most influential factor of ultrastructural quality, numerous other factors may also influence tissue preservation. The goal of the present study was to assess the effects of pre- and postmortem factors on multiple components of ultrastructure in the postmortem human prefrontal cortex. Tissue samples from 30 subjects were processed using standard EM histochemistry. The primary dependent measure was number of identifiable neuronal profiles, and secondary measures included presence and/or integrity of synapses, mitochondria, and myelinated axonal fibers. Number of identifiable neuronal profiles was most strongly affected by the interaction of PMI and pH, such that short PMIs and neutral pH values predicted the best preservation. Secondary measures were largely unaffected by pre- and postmortem factors. Together, these data indicate that distinct components of the neuropil are differentially affected by PMI and pH in postmortem human brain.
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