ArticleBiomolecular detection and quantification2018
Algorithms for automated detection of hook effect-bearing amplification curves.
Article in Biomolecular detection and quantification, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Automated post-run analysis of arrayed quantitative PCR amplification curves using machine learning.Gates open research · 2025Article
- Are the results from a multiplex proteomic assay and a conventional immunoassay for NT-proBNP and GDF-15 comparable?Clinical proteomics · 2023Article
- Cleavable molecular beacon-based loop-mediated isothermal amplification assay for the detection of adulterated chicken in meat.Analytical and bioanalytical chemistry · 2022Article
- Adaptive Filtering Framework to Remove Nonspecific and Low-Efficiency Reactions in Multiplex Digital PCR Based on Sigmoidal Trends.Analytical chemistry · 2022Article
- Article
- AScall - Automatic Allele-Specific qPCR Analysis.Frontiers in bioengineering and biotechnology · 2020Article
- qPCR data analysis: Better results through iconoclasm.Biomolecular detection and quantification · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amplification curves from quantitative Real-Time PCR experiments typically exhibit a sigmoidal shape. They can roughly be divided into a ground or baseline phase, an exponential amplification phase, a linear phase and finally a plateau phase, where in the latter, the PCR product concentration no longer increases. Nevertheless, in some cases the plateau phase displays a negative trend, e.g. in hydrolysis probe assays. This cycle-to-cycle fluorescence decrease is commonly referred to in the literature as the
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.