ArticleScientific reports2018
Transcriptome profiling of human oocytes experiencing recurrent total fertilization failure.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- Overview of Gene Expression Dynamics during Human Oogenesis/Folliculogenesis.International journal of molecular sciences · 2023Pooled it
- Single-cell analysis comparing early-stage oocytes from fresh and slow-frozen/thawed human ovarian cortex reveals minimal impact of cryopreservation on the oocyte transcriptome.Human reproduction (Oxford, England) · 2025Article
- DDX49 as a novel prognostic biomarker regulates colorectal cancer cell proliferation through the TIMM44-PI3K-AKT pathway.Frontiers in oncology · 2025Article
- RNA helicase DEAD-box-5 is involved in R-loop dynamics of preimplantation embryos.Animal bioscience · 2024Article
- A case report of a family with developmental arrest of human prokaryotic stage zygote.Frontiers in cell and developmental biology · 2024Article
- Single-cell RNA-sequencing of retrieved human oocytes and eggs in clinical practice and for human ovarian cell atlasing.Molecular reproduction and development · 2022Review
- Human embryonic genome activation initiates at the one-cell stage.Cell stem cell · 2022Article
- Clinical Relevance of Secreted Small Noncoding RNAs in an Embryo Implantation Potential Prediction at Morula and Blastocyst Development Stages.Life (Basel, Switzerland) · 2021Article
- The Spiny Mouse-A Menstruating Rodent to Build a Bridge From Bench to Bedside.Frontiers in reproductive health · 2021Review
- The Application of Single-Cell RNA Sequencing in Mammalian Meiosis Studies.Frontiers in cell and developmental biology · 2021Review
- Identifying the Translatome of Mouse NEBD-Stage Oocytes via SSP-Profiling; A Novel Polysome Fractionation Method.International journal of molecular sciences · 2020Article
- Genetics of human female infertility†.Biology of reproduction · 2019Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
There exist some patients who face recurrent total fertilization failure during assisted reproduction treatment, but the pathological mechanism underlying is elusive. Here, by using sc-RNA-seq method, the transcriptome profiles of ten abnormally fertilized zygotes were assessed, including five zygotes from one patient with recurrent Poly-PN zygotes, and five zygotes from a patient with pronuclear fusion failure. Four zygotes with three pronuclear (Tri-PN) were collected from four different patients as controls. After that, we identified 951 and 1697 significantly differentially expressed genes (SDEGs) in Poly-PN and PN arrest zygotes, respectively as compared with the control group. KEGG analyses indicated down regulated genes in the Poly-PN group included oocyte meiosis related genes, such as PPP2R1B, YWHAZ, MAD2L1, SPDYC, SKP1 and CDC27, together with genes associated with RNA processing, such as SF3B1, LOC645691, MAGOHB, PHF5A, PRPF18, DDX5, THOC1 and BAT1. In contrast, down regulated genes in the PN arrest group, included cell cycle genes, such as E2F4, DBF4, YWHAB, SKP2, CDC23, SMC3, CDC25A, CCND3, BUB1B, MDM2, CCNA2 and CDC7, together with homologous recombination related genes, such as NBN, XRCC3, SHFM1, RAD54B and RAD51. Thus, our work provides a better understanding of transcriptome profiles underlying RTFF, although it based on a limited number of patients.
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Registered trials
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