Evidence map›Paper›PMID 30559363›Full record

ArticleNature communications2018

Differentiation of primate primordial germ cell-like cells following transplantation into the adult gonadal niche.

Enrique Sosa, Di Chen, Ernesto J Rojas, Jon D Hennebold, Karen A Peters, Zhuang Wu, Truong N Lam, Jennifer M Mitchell, Meena Sukhwani, Ramesh C Tailor and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 76 citations in OpenAlex.

  1. Review
  2. Review
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  8. Non-human primate: the new frontier model of female reproductive engineering.Frontiers in bioengineering and biotechnology · 2025
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Enrique SosaDepartment of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Di ChenDepartment of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Ernesto J RojasDepartment of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Jon D HenneboldDivision of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR, 97006, USA.
Karen A PetersDepartment of Obstetrics, Gynecology and Reproductive Sciences and Magee Women's Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15213, USA.
Zhuang WuDepartment of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.
Truong N LamDepartment of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.
Jennifer M MitchellDepartment of Veterinary Medicine and Surgery, University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.
Meena SukhwaniDepartment of Obstetrics, Gynecology and Reproductive Sciences and Magee Women's Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15213, USA.
Ramesh C TailorDepartment of Radiation Physics, University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.
Marvin L MeistrichDepartment of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.
Kyle E OrwigDepartment of Obstetrics, Gynecology and Reproductive Sciences and Magee Women's Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15213, USA.
Gunapala ShettyDepartment of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.
Amander T ClarkDepartment of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, 90095, USA. clarka@ucla.edu.ORCID http://orcid.org/0000-0003-2483-3982
The University of Texas MD Anderson Cancer Center · USUniversity of California, Los Angeles · USUniversity of Pittsburgh · USOregon National Primate Research Center · US

Funding

Upgrade of confocal microscopy at the Oregon National Primate Research CenterP51OD011092 · OD · OREGON HEALTH & SCIENCE UNIVERSITY · PI Bonnie J. Nagel · 2012 to 2026
$203.9M
LABORATORY OF DEVELOPMENTAL BIOLOGYR24HD000836 · NICHD · UNIVERSITY OF WASHINGTON · PI Ian Amos Glass · 1995 to 2026
$16.9M
UCLA MSD ProgramR25GM055052 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BARBER, PAUL HENRY, HASSON, TAMA W. · 1996 to 2021
$9.2M
Transplant CoreP01HD075795 · NICHD · MAGEE-WOMEN'S RES INST AND FOUNDATION · PI ORWIG, KYLE EDWIN · 2014 to 2018
$7.0M
NICHD NIH HHS P01 HD075795NICHD NIH HHS R24 HD000836NIGMS NIH HHS R25 GM055052NIH HHS P51 OD011092
6 · The paper itself

Abstract

A major challenge in stem cell differentiation is the availability of bioassays to prove cell types generated in vitro are equivalent to cells in vivo. In the mouse, differentiation of primordial germ cell-like cells (PGCLCs) from pluripotent cells was validated by transplantation, leading to the generation of spermatogenesis and to the birth of offspring. Here we report the use of xenotransplantation (monkey to mouse) and homologous transplantation (monkey to monkey) to validate our in vitro protocol for differentiating male rhesus (r) macaque PGCLCs (rPGCLCs) from induced pluripotent stem cells (riPSCs). Specifically, transplantation of aggregates containing rPGCLCs into mouse and nonhuman primate testicles overcomes a major bottleneck in rPGCLC differentiation. These findings suggest that immature rPGCLCs once transplanted into an adult gonadal niche commit to differentiate towards late rPGCs that initiate epigenetic reprogramming but do not complete the conversion into ENO2-positive spermatogonia.

Indexed as

AnimalsCells, CulturedFemaleHumansInduced Pluripotent Stem CellsMacaca mulattaMaleMiceMice, NudeNeoplasm ProteinsPhosphopyruvate HydrataseSpermatocytesSpermatogenesisSpermatogoniaTestisTransplantation, HeterologousNeoplasm ProteinsPhosphopyruvate Hydratase

Identifiers

PMID30559363
PMCPMC6297357
OpenAlexW2772187133

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.