ArticleBiophysical journal2019
Convection-Induced Biased Distribution of Actin Probes in Live Cells.
Article in Biophysical journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics.iScience · 2025Article
- Multiple Mechanisms to Regulate Actin Functions: "Fundamental" Versus Lineage-Specific Mechanisms and Hierarchical Relationships.Biomolecules · 2025Review
- Moesin integrates cortical and lamellar actin networks during Drosophila macrophage migration.Nature communications · 2025Article
- Designed Ankyrin Repeat Proteins as Actin Labels of Distinct Cytoskeletal Structures in Living Cells.ACS nano · 2024Article
- Force transmission by retrograde actin flow-induced dynamic molecular stretching of Talin.Nature communications · 2023Article
- Fidgetin-like 2 depletion enhances cell migration by regulating GEF-H1, RhoA, and FAK.Biophysical journal · 2023Article
- A two-step search and run response to gradients shapes leukocyte navigation in vivo.The Journal of cell biology · 2022Article
- Evaluation of Stable LifeAct-mRuby2- and LAMP1-NeonGreen Expressing A549 Cell Lines for Investigation ofInternational journal of molecular sciences · 2021Article
- Biased localization of actin binding proteins by actin filament conformation.Nature communications · 2020Article
- Highly photostable fluorescent labeling of proteins in live cells using exchangeable coiled coils heterodimerization.Cellular and molecular life sciences : CMLS · 2020Article
- Spatiotemporal monitoring of intracellular metabolic dynamics by resonance Raman microscopy with isotope labeling.RSC advances · 2020Article
- Quantitative high-precision imaging of myosin-dependent filamentous actin dynamics.Journal of muscle research and cell motility · 2020Review
- Lamellipodium tip actin barbed ends serve as a force sensor.Genes to cells : devoted to molecular & cellular mechanisms · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Fluorescent markers that bind endogenous target proteins are frequently employed for quantitative live-cell imaging. To visualize the actin cytoskeleton in live cells, several actin-binding probes have been widely used. Among them, Lifeact is the most popular probe with ideal properties, including fast exchangeable binding kinetics. Because of its fast kinetics, Lifeact is generally believed to distribute evenly throughout cellular actin structures. In this study, however, we demonstrate misdistribution of Lifeact toward the rear of lamellipodia where actin filaments continuously move inward along the retrograde flow. Similarly, phalloidin showed biased misdistribution toward the rear of lamellipodia in live cells. We show evidence of convection-induced misdistribution of actin probes by both experimental data and physical models. Our findings warn about the potential error arising from the use of target-binding probes in quantitative live imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.