ArticleBMC plant biology2018
Proline synthesis in developing microspores is required for pollen development and fertility.
Article in BMC plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- Trifluoromethanesulfonamide Induces Male Sterility Through Systemic Metabolic Reprogramming and Anther-Specific Proline Deficiency.International journal of molecular sciences · 2026Article
- Beyond osmoprotection: the expanding roles of proline in plant signalling and development.Biologia futura · 2026Review
- ABA-Induced Transcriptomic Dynamics inPlants (Basel, Switzerland) · 2026Article
- Adapting Crops to Rising Temperatures: Understanding Heat Stress and Plant Resilience Mechanisms.International journal of molecular sciences · 2025Review
- Extreme heat affects blueberry pollen nutrition, bee health, and plant reproduction.Scientific reports · 2025Article
- Pollen and morphometric analysis revealPhytoKeys · 2025Article
- Proline and ROS: A Unified Mechanism in Plant Development and Stress Response?Plants (Basel, Switzerland) · 2024Review
- Proline Metabolism Genes in Transgenic Plants: Meta-Analysis under Drought and Salt Stress.Plants (Basel, Switzerland) · 2024Article
- Disruption of the Contents of Endogenous Hormones Cause Pollen Development Obstruction and Abortion in Male-Sterile Hybrid Lily Populations.Plants (Basel, Switzerland) · 2023Article
- A Systematic Investigation of Lipid Transfer Proteins Involved in Male Fertility and Other Biological Processes in Maize.International journal of molecular sciences · 2023Article
- Interplay between Proline Metabolism and ROS in the Fine Tuning of Root-Meristem Size inPlants (Basel, Switzerland) · 2022Article
- Functions of Redox Signaling in Pollen Development and Stress Response.Antioxidants (Basel, Switzerland) · 2022Review
- Intriguing Role of Proline in Redox Potential Conferring High Temperature Stress Tolerance.Frontiers in plant science · 2022Review
- HalophyteFrontiers in plant science · 2022Article
- Differential reward in "male" versus "female" pollen of functionally dioecious Solanum.American journal of botany · 2021Article
- Editorial: Amino Acids in Plants: Regulation and Functions in Development and Stress Defense.Frontiers in plant science · 2021Article
- Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile LineFrontiers in plant science · 2021Article
- Review
- Proline Accumulation in Pollen Grains as Potential Target for Improved Yield Stability Under Salt Stress.Frontiers in plant science · 2020Article
- Differential Contribution of P5CS Isoforms to Stress Tolerance in Arabidopsis.Frontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundIn many plants, the amino acid proline is strongly accumulated in pollen and disruption of proline synthesis caused abortion of microspore development in Arabidopsis. So far, it was unclear whether local biosynthesis or transport of proline determines the success of fertile pollen development.
resultsWe analyzed the expression pattern of the proline biosynthetic genes PYRROLINE-5-CARBOXYLATE SYNTHETASE 1 & 2 (P5CS1 & 2) in Arabidopsis anthers and both isoforms were strongly expressed in developing microspores and pollen grains but only inconsistently in surrounding sporophytic tissues. We introduced in a p5cs1/p5cs1 p5cs2/P5CS2 mutant background an additional copy of P5CS2 under the control of the Cauliflower Mosaic Virus (CaMV) 35S promoter, the tapetum-specific LIPID TRANSFER PROTEIN 12 (Ltp12) promoter or the pollen-specific At5g17340 promoter to determine in which site proline biosynthesis can restore the fertility of proline-deficient microspores. The specificity of these promoters was confirmed by β-glucuronidase (GUS) analysis, and by direct proline measurement in pollen grains and stage-9/10 anthers. Expression of P5CS2 under control of the At5g17340 promoter fully rescued proline content and normal morphology and fertility of mutant pollen. In contrast, expression of P5CS2 driven by either the Ltp12 or CaMV35S promoter caused only partial restoration of pollen development with little effect on pollen fertility.
conclusionsOverall, our results indicate that proline transport is not able to fulfill the demand of the cells of the male germ line. Pollen development and fertility depend on local proline biosynthesis during late stages of microspore development and in mature pollen grains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.