ArticleBMC genomics2018
Identification and characterisation of microRNAs and their target genes in phosphate-starved Nicotiana benthamiana by small RNA deep sequencing and 5'RACE analysis.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The role of microRNAs in responses to drought and heat stress in peanut (Arachis hypogaea).The plant genome · 2023Pooled it
- Analysing the loss of embryogenic competence in long-term cell lines of Solanum betaceum Cav.: involvement of miR827, phosphate and sugar.BMC plant biology · 2025Article
- Differential miRNA expression and regulatory mechanisms in pigmentation and fiber development of white and brown cotton (Gossypium hirsutum).Functional & integrative genomics · 2025Article
- A multi-omic Nicotiana benthamiana resource for fundamental research and biotechnology.Nature plants · 2023Article
- INDETERMINATE1 autonomously regulates phosphate homeostasis upstream of the miR399-ZmPHO2 signaling module in maize.The Plant cell · 2023Article
- Functional Analysis of Bna-miR399c-Life (Basel, Switzerland) · 2023Article
- Plants Recruit Peptides and Micro RNAs to Regulate Nutrient Acquisition from Soil and Symbiosis.Plants (Basel, Switzerland) · 2023Review
- Functional role of microRNA in the regulation of biotic and abiotic stress in agronomic plants.Frontiers in genetics · 2023Review
- Insights into Mobile Small-RNAs Mediated Signaling in Plants.Plants (Basel, Switzerland) · 2022Review
- MicroRNA398: A Master Regulator of Plant Development and Stress Responses.International journal of molecular sciences · 2022Review
- The Mobile Small RNAs: Important Messengers for Long-Distance Communication in Plants.Frontiers in plant science · 2022Review
- Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses.BMC genomics · 2021Article
- Systemic Optimization of Legume Nodulation: A Shoot-Derived Regulator, miR2111.Frontiers in plant science · 2021Review
- Transcriptional, metabolic, physiological and developmental responses of switchgrass to phosphorus limitation.Plant, cell & environment · 2021Article
- Genome-wide identification of low phosphorus responsive microRNAs in two soybean genotypes by high-throughput sequencing.Functional & integrative genomics · 2020Article
- MIR2111-5 locus and shoot-accumulated mature miR2111 systemically enhance nodulation depending on HAR1 in Lotus japonicus.Nature communications · 2020Article
- sRNATargetDigger: A bioinformatics software for bidirectional identification of sRNA-target pairs with co-regulatory sRNAs information.PloS one · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
backgroundPhosphorus is an important macronutrient that is severely lacking in soils. In plants, specific microRNAs (miRNAs) essential for nutrient management and the regulation of stress responses are responsible for the control of many phosphate starvation responses. Further understanding of conserved and species-specific microRNA species has potential implications for the development of crops tolerant to soils with low phosphate.
resultsThis study identified and characterised phosphate starvation-responsive miRNAs in the native Australian tobacco Nicotiana benthamiana. Small RNA libraries were constructed and sequenced from phosphate-starved plant leaves, stems and roots. Twenty-four conserved miRNA families and 36 species-specific miRNAs were identified. The majority of highly phosphate starvation-responsive miRNAs were highly conserved, comprising of members from the miR399, miR827, and miR2111 families. In addition, two miRNA-star species were identified to be phosphate starvation-responsive. A total of seven miRNA targets were confirmed using RLM-5'RACE to be cleaved by five miRNA families, including two confirmed cleavage targets for Nbe-miR399 species, one for Nbe-miR2111, and two for Nbe-miR398. A number of N. benthamiana-specific features for conserved miRNAs were identified, including species-specific miRNA targets predicted or confirmed for miR399, miR827, and miR398.
conclusionsOur results give an insight into the phosphate starvation-responsive miRNAs of Nicotiana benthamiana, and indicate that the phosphate starvation response pathways in N. benthamiana contain both highly conserved and species-specific components.
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