ArticlePloS one2018
Systematic in vitro comparison of decellularization protocols for blood vessels.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
57 citing papers in PubMed, 112 citations in OpenAlex.
- Decellularized matrix grafts and peripheral nerve regeneration.Neural regeneration research · 2026Article
- Tissue compliance characterization for human umbilical arteries after decellularization.Journal of the mechanical behavior of biomedical materials · 2026Article
- Detergent-Based Decellularization Preserves Extracellular Matrix Ultrastructure in Ovine Soft Tissues.Biomimetics (Basel, Switzerland) · 2026Article
- Vascularized Cardiac Tissue Engineering: From Advances in Biofabrication to Translational Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Bioactive and Injectable Granular Hydrogels Incorporating Decellularized Extracellular Matrix.ACS biomaterials science & engineering · 2026Article
- Proteomic evaluation and comparison of native and decellularized human saphenous vein extracellular matrices.Frontiers in bioengineering and biotechnology · 2026Article
- Fabrication and characterization of decellularized human endometrial hydrogel as a scaffold for in vitro embryo implantation: An experimental study.International journal of reproductive biomedicine · 2026Article
- Lifting regenerative barriers promotes epithelial cell fate plasticity supporting lineage conversion.Nature communications · 2025Article
- Determination of residual DNA in decellularised aortas- towards fluorescence-based quantification of DNA purified by various methods.Molecular biology reports · 2025Article
- Decellularized Porcine Aorta as a Scaffold for Human Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells in Tissue Engineering.Stem cell reviews and reports · 2025Article
- Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations.Exploration (Beijing, China) · 2025Review
- Organoids technology in cancer research: from basic applications to advancedFrontiers in cell and developmental biology · 2025Review
- A pilot study for decellularizing porcine cornea for future use in corneal regeneration.PloS one · 2025Article
- Decellularized Green and Brown Macroalgae as Cellulose Matrices for Tissue Engineering.Journal of functional biomaterials · 2024Article
- Decellularization of various tissues and organs through chemical methods.Tissue & cell · 2024Review
- Article
- Decellularized kidney capsule as a three-dimensional scaffold for tissue regeneration.Cell and tissue banking · 2024Article
- Three-Dimensional Cultivation a Valuable Tool for Modelling Canine Mammary Gland Tumour Behaviour In Vitro.Cells · 2024Review
- Proteome of Personalized Tissue-Engineered Veins.ACS omega · 2024Article
- An overview of the production of tissue extracellular matrix and decellularization process.Cell and tissue banking · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Decellularization of native blood vessels is a promising technology to generate 3D biological scaffolds for vascular grafting. Blood vessel decellularization has been performed in previous studies under various experimental conditions, that complicates comparison and optimization of suitable protocols. The goal of this work was to systematically compare the decellularization and recellularization efficacy of 5 different protocols utilizing the detergents sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC), CHAPS and TritonX-100 together with DNA-removing enzymes on porcine vena cava in a perfusion bioreactor setup. Additionally, we tested the effect of DNase on the extracellular matrix (ECM) properties. We found that all protocols could efficiently decellularize blood vessels. Mechanical strength, collagen preservation and ECM integrity were similar among all tested detergents, yet TritonX protocols required long-term DNase application for complete decellularization. However, TritonX-based protocols showed the greatest recellularization efficacy with HUVECs in vitro. Furthermore, we developed a novel protocol for TritonX which improved recellularization and reduced total process time and ECM stiffness compared to previous protocols. SDS, SDC and CHAPS based protocols had a lower recellularization potential. In conclusion, decellularization of blood vessels can be achieved with all tested reagents, but TritonX treated ECM can be most efficiently recellularized with endothelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.