Evidence map›Paper›PMID 30556645›Full record

ArticleCurrent protocols in cytometry2019

Flow Cytometry Analysis of Free Intracellular NAD

Jared M Eller, Melissa L Stewart, Alexandria J Slepian, Sheila Markwardt, Jack Wiedrick, Michael S Cohen, Richard H Goodman, Xiaolu A Cambronne

Abstract read
In one paragraph

Article in Current protocols in cytometry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Mitochondrial NADScience advances · 2025
    Article
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Jared M EllerDepartment of Molecular Biosciences, University of Texas at Austin, Austin, Texas.
Melissa L StewartVollum Institute, Oregon Health & Science University, Portland, Oregon.
Alexandria J SlepianVollum Institute, Oregon Health & Science University, Portland, Oregon.
Sheila MarkwardtBiostatistics and Design Program, Oregon Health & Science University, Portland, Oregon.
Jack WiedrickBiostatistics and Design Program, Oregon Health & Science University, Portland, Oregon.
Michael S CohenDepartment of Physiology and Pharmacology, Oregon Health & Science University, Portland, Oregon.
Richard H GoodmanVollum Institute, Oregon Health & Science University, Portland, Oregon.
Xiaolu A CambronneDepartment of Molecular Biosciences, University of Texas at Austin, Austin, Texas.
Oregon Health & Science University · USVollum Institute · USThe University of Texas at Austin · US

Funding

Understanding the origins of rapid recurrence of pancreatic cancer after resectionP30CA069533 · NCI · OREGON HEALTH & SCIENCE UNIVERSITY · PI Luiz Eduardo Bertassoni · 1997 to 2026
$60.5M
Oregon Clinical and Translational Research InstituteUL1TR000128 · NCATS · OREGON HEALTH & SCIENCE UNIVERSITY · PI ELLISON, DAVID H · 2012 to 2015
$28.7M
Decoding protein MARylation networks in astrocytes using chemical biology approachesR01NS088629 · NINDS · OREGON HEALTH & SCIENCE UNIVERSITY · PI COHEN, MICHAEL S · 2014 to 2024
$4.2M
Gatekeepers of Mitochondrial NAD+DP2GM126897 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI CAMBRONNE, XIAOLU ANG · 2017 to 2021
$2.4M
Monitoring NAD+ levels in aging using a novel genetically-encoded biosensorR01AG055431 · NIA · OREGON HEALTH & SCIENCE UNIVERSITY · PI GOODMAN, RICHARD H. · 2017 to 2020
$1.5M
NCATS NIH HHS UL1 TR000128NCI NIH HHS P30 CA069533NIA NIH HHS R01 AG055431NIGMS NIH HHS DP2 GM126897NINDS NIH HHS R01 NS088629
6 · The paper itself

Abstract

Flow cytometry approaches combined with a genetically encoded targeted fluorescent biosensor are used to determine the subcellular compartmental availability of the oxidized form of nicotinamide adenine dinucleotide (NAD

Indexed as

AcrylamidesBiosensing TechniquesCalibrationDigitoninEnzyme InhibitorsFlow CytometryFluorescenceHeLa CellsHumansHydrogen-Ion ConcentrationIntracellular SpaceMitochondriaNADNicotinamide PhosphoribosyltransferasePiperidinesSmall Molecule LibrariesAcrylamidesDigitoninEnzyme InhibitorsN-(4-(1-benzoylpiperidin-4-yl)butyl)-3-(pyridin-3-yl)acrylamideNADNicotinamide PhosphoribosyltransferasePiperidinesSmall Molecule LibrariesARTDCD-38circularly permutated fluorescent proteinfluorescent sensorgenetically encoded biosensormetaboliteNAD+nicotinamide adenine dinucleotidePARPsirtuin

Identifiers

PMID30556645
PMCPMC6422705
OpenAlexW2905537334

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.