Evidence map›Paper›PMID 30556148›Full record

ArticleElectrophoresis2019

Electrophoresis of megaDalton proteins inside colloidal silica.

Tamika S Ragland, Melody D Gossage, Michael D Furtaw, Jon P Anderson, David L Steffens, Mary J Wirth

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Electrophoresis, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Tamika S RaglandDepartment of Chemistry, Purdue University, West Lafayette, IN, USA.
Melody D GossageEli Lilly and Company, Indianapolis, IN, USA.
Michael D FurtawLI-COR Biosciences, Inc., Lincoln, NE, USA.
Jon P AndersonLI-COR Biosciences, Inc., Lincoln, NE, USA.
David L SteffensLI-COR Biosciences, Inc., Lincoln, NE, USA.
Mary J WirthDepartment of Chemistry, Purdue University, West Lafayette, IN, USA.ORCID 0000-0003-2538-6985

Funding

Sub-1 um particles for nanoLCMS of intact proteinsR01GM121910 · NIGMS · PURDUE UNIVERSITY · PI HRYCYNA, CHRISTINE A · 2017 to 2020
$1.1M
Submicrometer silica particles for high-throughput separations of protein pharmacR01GM101464 · NIGMS · PURDUE UNIVERSITY · PI WIRTH, MARY J. · 2012 to 2015
$1.1M
LICOR BiosciencesLilly Research Award ProgramNIGMS NIH HHS R01 GM101464NIGMS NIH HHS R01 GM121910NIH HHS R01GM121910
6 · The paper itself

Abstract

With the growth of the biopharmaceutical industry, there is a need for rapid size-analysis of proteins on the megaDalton scale. The large pore sizes needed for such separations cannot be easily reached by pushing the current limits of size-exclusion chromatography or gel electrophoresis. The concept detailed here is the formation of arbitrarily wide pores by packing nonporous colloidal silica in capillaries. This method can be called packed-capillary electrophoresis, or "pCE". Electrophoresis of protein standards (11-155 kDa) by pCE, using 345 nm diameter particles in 100 μm diameter capillaries, gives 2x higher resolution than a typical PAGE gel in 1/6 of the time. The electropherograms show that pCE is highly efficient, with half-micrometer plate heights for all seven standards, giving 10

Indexed as

Recombinant ProteinsColloidsElectrophoresis, CapillaryParticle SizePorositySilicon DioxideColloidsRecombinant ProteinsSilicon DioxideAggregationComplexesIgGMDaSeparation

Identifiers

PMID30556148
PMCPMC6711471

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.