ArticleBiotechnology for biofuels2018
Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green alga
Article in Biotechnology for biofuels, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 62 citations in OpenAlex.
- Highly efficient genome editing using CRISPR/Cas9 ribonucleoprotein in the marine oleaginous diatom Fistulifera solaris.Scientific reports · 2026Article
- Pre-mRNA splicing and its regulation in microalgae and cyanobacteria.Advanced biotechnology · 2025Review
- Microalgal biorefineries: a systematic review of technological trade-offs and innovation pathways.Biotechnology for biofuels and bioproducts · 2025Review
- Development of a CRISPR/Cas9-mediated gene-editing method to isolate a mutant of the unicellular green alga Parachlorella kessleri strain NIES-2152 with improved lipid productivity.Biotechnology for biofuels and bioproducts · 2024Article
- High-efficiency genome editing by Cas12a ribonucleoprotein complex in Euglena gracilis.Microbial biotechnology · 2024Article
- Effects of two types of Coccomyxa sp. KJ on in vitro ruminal fermentation, methane production, and the rumen microbiota.PloS one · 2024Article
- DANGER analysis: risk-averse on/off-target assessment for CRISPR editing without a reference genome.Bioinformatics advances · 2023Article
- Genome editing with removable TALEN vectors harboring a yeast centromere and autonomous replication sequence in oleaginous microalga.Scientific reports · 2022Article
- Article
- Development of a species-specific transformation system using the novel endogenous promoter calreticulin from oleaginous microalgae Ettlia sp.Scientific reports · 2020Article
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Genetic Engineering Strategies forFrontiers in bioengineering and biotechnology · 2020Review
- CRISPR-Cas9 System for Genome Engineering of Photosynthetic Microalgae.Molecular biotechnology · 2019Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOxygen-evolving photosynthetic microorganisms, collectively termed as microalgae, are gaining attention as alternative fuel sources. The unicellular alga
resultsIn this paper, we report successful genome editing of strain KJ by intracellular delivery of a ribonucleoprotein complex comprising recombinant Cas9 protein and guide RNA. For introduction of Cas9-guide RNA ribonucleoprotein into strain KJ cells, we used an electroporator with a short (2.5 ms) electric pulse at a high field strength (7500 V cm
conclusionsOur study shows applicability of DNA-free genome editing in
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