Evidence map›Paper›PMID 30555356›Full record

ArticleFrontiers in physiology2018

Conditioned Media From Glial Cells Promote a Neural-Like Connexin Expression in Human Adipose-Derived Mesenchymal Stem Cells.

Debora Lo Furno, Giuliana Mannino, Rosalia Pellitteri, Agata Zappalà, Rosalba Parenti, Elisa Gili, Carlo Vancheri, Rosario Giuffrida

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 23 citations in OpenAlex.

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  11. The Role of Epigenetics in Neuroinflammatory-Driven Diseases.International journal of molecular sciences · 2022
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  12. Review
  13. Bio-Scaffolds as Cell or Exosome Carriers for Nerve Injury Repair.International journal of molecular sciences · 2021
    Review
  14. Article
  15. In focus in HCB.Histochemistry and cell biology · 2021
    Article
  16. Article
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  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Debora Lo FurnoDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Catania, Italy.
Giuliana ManninoDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Catania, Italy.
Rosalia PellitteriInstitute of Neurological Sciences, National Research Council, Catania, Italy.
Agata ZappalàDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Catania, Italy.
Rosalba ParentiDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Catania, Italy.
Elisa GiliDepartment of Clinical and Experimental Medicine, University of Catania, Catania, Italy.
Carlo VancheriDepartment of Clinical and Experimental Medicine, University of Catania, Catania, Italy.
Rosario GiuffridaDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Catania, Italy.
University of Catania · ITNational Research Council · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The expression of neuronal and glial connexins (Cxs) has been evaluated in adipose-derived mesenchymal stem cells (ASCs) whose neural differentiation was promoted by a conditioned medium (CM) obtained from cultures of olfactory ensheathing cells (OECs) or Schwann cells (SCs). By immunocytochemistry and flow cytometer analysis it was found that Cx43 was already considerably expressed in naïve ASCs and further increased after 24 h and 7 days from CM exposition. Cx32 and Cx36 were significantly improved in conditioned cultures compared to control ASCs, whereas a decreased expression was noticed in the absence of CM treatments. Cx47 was virtually absent in any conditions. Altogether, high basal levels and induced increases of Cx43 expression suggest a potential attitude of ASCs toward an astrocyte differentiation, whereas the lack of Cx47 would indicate a poor propensity of ASCs to become oligodendrocytes. CM-evoked Cx32 and Cx36 increases showed that a neuronal- or a SC-like differentiation can be promoted by using this strategy. Results further confirm that environmental cues can favor an ASC neural differentiation, either as neuronal or glial elements. Of note, the use of glial products present in CM rather than the addition of chemical agents to achieve such differentiation would resemble "more physiological" conditions of differentiation. As a conclusion, the overexpression of typical neural Cxs would indicate the potential capability of neural-like ASCs to interact with neighboring neural cells and microenvironment.

Indexed as

connexin expressionglial conditioned mediahuman adipose mesenchymal stem cellsneural-like differentiationolfactory ensheathing cellsSchwann cells

Identifiers

PMID30555356
PMCPMC6282092
OpenAlexW2902714381

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.