ArticleJournal of industrial microbiology & biotechnology2019
Improved cell growth and biosynthesis of glycolic acid by overexpression of membrane-bound pyridine nucleotide transhydrogenase.
Article in Journal of industrial microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 16 citations in OpenAlex.
- Integrated amplification of NADPH-regenerating modules enhances cytidine biosynthesis inSynthetic and systems biotechnology · 2026Article
- Cofactor engineering for improved production of 2,4-dihydroxybutyric acid via the synthetic homoserine pathway.Frontiers in bioengineering and biotechnology · 2025Article
- Exploring yeast biodiversity and process conditions for optimizing ethylene glycol conversion into glycolic acid.FEMS yeast research · 2024Article
- Ferulic acid production by metabolically engineered Escherichia coli.Bioresources and bioprocessing · 2021Article
- Understanding D-xylonic acid accumulation: a cornerstone for better metabolic engineering approaches.Applied microbiology and biotechnology · 2021Review
- Enhanced glycolic acid yield through xylose and cellobiose utilization by metabolically engineered Escherichia coli.Bioprocess and biosystems engineering · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The non-conventional D-xylose metabolism called the Dahms pathway which only requires the expression of at least three enzymes to produce pyruvate and glycolaldehyde has been previously engineered in Escherichia coli. Strains that rely on this pathway exhibit lower growth rates which were initially attributed to the perturbed redox homeostasis as evidenced by the lower intracellular NADPH concentrations during exponential growth phase. NADPH-regenerating systems were then tested to restore the redox homeostasis. The membrane-bound pyridine nucleotide transhydrogenase, PntAB, was overexpressed and resulted to a significant increase in biomass and glycolic acid titer and yield. Furthermore, expression of PntAB in an optimized glycolic acid-producing strain improved the growth and product titer significantly. This work demonstrated that compensating for the NADPH demand can be achieved by overexpression of PntAB in E. coli strains assimilating D-xylose through the Dahms pathway. Consequently, increase in biomass accumulation and product concentration was also observed.
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Registered trials
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