ArticleDevelopmental biology2019
Live imaging reveals hub cell assembly and compaction dynamics during morphogenesis of the Drosophila testis niche.
Article in Developmental biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 27 citations in OpenAlex.
- Signaling pathways in Drosophila testis niche: Local signals that regulate stem cell fate.Insect science · 2026Review
- Harnessing the Power ofCold Spring Harbor perspectives in biology · 2026Review
- Lin28 Represses Hub Cell microRNAs to MaintainDevelopment & reproduction · 2026Review
- The Tbx1 ortholog org-1 is required to establish testis stem cell niche identity in Drosophila.Development (Cambridge, England) · 2026Article
- Plexin/Semaphorin antagonism orchestrates collective cell migration and organ sculpting by regulating epithelial-mesenchymal balance.Science advances · 2025Article
- miR-932 Suppresses the Expression of Germline-SpecificDevelopment & reproduction · 2025Article
- TheeLife · 2025Article
- Maintenance of niche architecture requires actomyosin and enables proper stem cell signaling and oriented division in the Drosophila testis.Development (Cambridge, England) · 2025Article
- Generation ofmicroPublication biology · 2025Article
- ThebioRxiv : the preprint server for biology · 2024Article
- An actomyosin network organizes niche morphology and responds to feedback from recruited stem cells.Current biology : CB · 2024Article
- An actomyosin network organizes niche morphology and responds to feedback from recruited stem cells.bioRxiv : the preprint server for biology · 2024Article
- Lin28 is Required for Single Niche Development in theDevelopment & reproduction · 2023Article
- Kinetics of blood cell differentiation during hematopoiesis revealed by quantitative long-term live imaging.eLife · 2023Article
- Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq ofeLife · 2023Article
- Insights and perspectives on the enigmatic alary muscles of arthropods.Frontiers in cell and developmental biology · 2023Review
- Live Imaging of the Drosophila Testis Stem Cell Niche.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Visceral mesoderm signaling regulates assembly position and function of the Drosophila testis niche.Developmental cell · 2022Article
- Proliferative stem cells maintain quiescence of their niche by secreting the Activin inhibitor Follistatin.Developmental cell · 2021Article
- Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad.Journal of visualized experiments : JoVE · 2020Article
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4 authors at 2 institutions in 1 country.
Funding
Abstract
Adult stem cells are often found in specialized niches, where the constituent cells direct self-renewal of their stem cell pool. The niche is therefore crucial for both normal homeostasis and tissue regeneration. In many mammalian tissues, niche cells have classically been difficult to identify, which has hampered any understanding of how tissues first construct niches during development. Fortunately, the Drosophila germline stem cell (GSC) niche is well defined, allowing for unambiguous identification of both niche cells and resident stem cells. The testis niche first forms in the early embryo, during a late stage of gonadogenesis. Here, using live-imaging both in vivo and ex vivo, we follow pro-niche cells as they assemble and assume their final form. We show that after ex vivo culture the niche appears fully functional, as judged by enrichment of adhesion proteins, the ability to activate STAT in adjacent GSCs, and to direct GSCs to divide orthogonally to the niche, just as they would in situ. Collectively, our imaging has generated several novel insights on niche morphogenesis that could not be inferred from fixed images alone. We identify dynamic processes that constitute an assembly phase and a compaction phase during morphogenesis. The compaction phase correlates with cell neighbor exchange among the assembled pro-niche cells, as well as a burst of divisions among newly recruited stem cells. Before compaction, an assembly phase involves the movement of pro-niche cells along the outer periphery of the gonad, using the extracellular matrix (ECM) to assemble at the anterior of the gonad. Finally, live-imaging in integrin mutants allows us to define the role of pro-niche cell-ECM interaction with regard to the new assembly and compaction dynamics revealed here.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.