ArticleScientific reports2018
Deep sequencing and transcriptome analysis to identify genes related to biosynthesis of aristolochic acid in Asarum heterotropoides.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed.
- Isolation and Characterization of Two Monoterpene Synthases and a Sesquiterpene Synthase fromMetabolites · 2025Article
- Heterologous biosynthesis of medicarpin using engineeredSynthetic and systems biotechnology · 2023Article
- Analysis of Floral Organ Development and Sex Determination inLife (Basel, Switzerland) · 2022Article
- Microbial degradation of aristolochic acid I by endophytic fungus A.h-Fs-1 ofFrontiers in microbiology · 2022Article
- Application of High-Throughput Sequencing on the Chinese Herbal Medicine for the Data-Mining of the Bioactive Compounds.Frontiers in plant science · 2022Review
- Transcriptional response of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. leaves grown under full and partial daylight conditions.BMC genomics · 2021Article
- Transcriptome Analysis ofInternational journal of molecular sciences · 2019Article
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4 authors.
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Abstract
Asarum spp. are important medicinal plants that have the potential for use in treating various types of fevers. Aristolochic acid is one of the main toxic compounds present in these plants. To improve our understanding of the biosynthetic pathway of aristolochic acid, we sequenced the transcriptome of the root and leaf tissues of Asarum heterotropoides and performed de novo sequence assembly. The data were stitched together to produce 468,357 transcripts with an N50 of 611 bp. The data were annotated with various databases (RefSeq non-redundant proteins [Nr], Swiss-Prot, Kyoto Encyclopaedia of Genes and Genomes [KEGG], Clusters of Orthologous Groups/EuKaryotic Orthologous Groups [COG/KOG], and Gene Ontology [GO]) and were annotated. There were 205,165 transcripts (43.81%) of differentially expressed genes in the roots and leaves, which were shown to be involved in biosynthesis, transport, and catabolism, and 100 genes in defence mechanisms. Three candidate transcripts (TyrDC1, TyrDC2, and TyrDC3) were discovered in these differential genes. TyrDC may be a key enzyme in the biosynthesis pathway of aristolochic acid identified using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and high-performance liquid chromatography (HPLC). The transcriptome data and analysis presented here lay the foundation for further research into these important medicinal plants.
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