ArticleScientific reports2018
Nanopore-based detection and characterization of yam viruses.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 82 citations in OpenAlex.
- Pooled it
- Advanced Spectroscopic, Imaging, and Nanotechnology Tools for Diagnosing Fungal Diseases in Fruits.Food science & nutrition · 2026Review
- Metagenomic screening of the virome of symptomatic tomato plants from La Réunion Island uncovers a complex of viruses including a newly identified whitefly-transmitted polerovirus.Archives of virology · 2026Article
- Review
- Article
- Review
- Deciphering mixed infections by plant RNA virus and reconstructing complete genomes simultaneously present within-host.PloS one · 2025Article
- Nanomaterial-based biosensors: a new frontier in plant pathogen detection and plant disease management.Frontiers in bioengineering and biotechnology · 2025Review
- Identification of Viruses InfectingInternational journal of molecular sciences · 2024Article
- Detection of Plant Viruses Using Nanopore Sequencing Based Metagenomic Approach.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Recent advances and challenges in plant viral diagnostics.Frontiers in plant science · 2024Review
- Nanopore-Sequencing Metabarcoding for Identification of Phytopathogenic and Endophytic Fungi in Olive (Journal of fungi (Basel, Switzerland) · 2023Article
- Evaluation of sequencing and PCR-based methods for the quantification of the viral genome formula.Virus research · 2023Article
- Emerging and Re-Emerging Diseases Caused by Badnaviruses.Pathogens (Basel, Switzerland) · 2023Review
- NanoViromics: long-read sequencing of dsRNA for plant virus and viroid rapid detection.Frontiers in microbiology · 2023Article
- Advancing the automation of plant nucleic acid extraction for rapid diagnosis of plant diseases in space.Frontiers in plant science · 2023Article
- Identification of an emerging cucumber virus in Taiwan using Oxford nanopore sequencing technology.Plant methods · 2022Article
- Molecular characterization of Cordyline virus 1 isolates infecting yam (Dioscorea spp).Archives of virology · 2022Article
- Epidemiology of Yam Viruses in Guadeloupe: Role of Cropping Practices and Seed-Tuber Supply.Viruses · 2022Article
- Nanopore Metagenomics Sequencing for Rapid Diagnosis and Characterization of Lily Viruses.The plant pathology journal · 2022Article
Corrections and comments
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Authors and funding
11 authors at 8 institutions in 4 countries.
Funding
Abstract
We here assessed the capability of the MinION sequencing approach to detect and characterize viruses infecting a water yam plant. This sequencing platform consistently revealed the presence of several plant virus species, including Dioscorea bacilliform virus, Yam mild mosaic virus and Yam chlorotic necrosis virus. A potentially novel ampelovirus was also detected by a complimentary Illumina sequencing approach. The full-length genome sequence of yam chlorotic necrosis virus was determined using Sanger sequencing, which enabled determination of the coverage and sequencing accuracy of the MinION technology. Whereas the total mean sequencing error rate of yam chlorotic necrosis virus-related MinION reads was 11.25%, we show that the consensus sequence obtained either by de novo assembly or after mapping the MinION reads on the virus genomic sequence was >99.8% identical with the Sanger-derived reference sequence. From the perspective of potential plant disease diagnostic applications of MinION sequencing, these degrees of sequencing accuracy demonstrate that the MinION approach can be used to both reliably detect and accurately sequence nearly full-length positive-sense single-strand polyadenylated RNA plant virus genomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.