ArticleThe Laryngoscope2019
Motor endplate-expressing cartilage-muscle implants for reconstruction of a denervated hemilarynx.
Article in The Laryngoscope, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Novel Biomolecule-Infused Gelatin Injectable for Treatment of Recurrent Laryngeal Nerve Injury.The Laryngoscope · 2025Article
- Multi-Layered Implant Approach for Hemilaryngectomy Reconstruction in a Porcine Model.The Laryngoscope · 2025Article
- Optimizing transport methods to preserve function of self-innervating muscle cells for laryngeal injection.Laryngoscope investigative otolaryngology · 2024Article
- Tissue-Engineered Implant for Hemilaryngectomy Reconstruction with Recurrent Laryngeal Nerve Injury.The Laryngoscope · 2024Article
- Early Changes in Porcine Larynges Following Injection of Motor-Endplate Expressing Muscle Cells for the Treatment of Unilateral Vocal Fold Paralysis.The Laryngoscope · 2024Article
- Biocompatible Materials in Otorhinolaryngology and Their Antibacterial Properties.International journal of molecular sciences · 2022Review
- Changes of Functional, Morphological, and Inflammatory Reactions in Spontaneous Peripheral Nerve Reinnervation after Thermal Injury.Oxidative medicine and cellular longevity · 2022Article
- Laryngeal Reconstruction Using Tissue-Engineered Implants in Pigs: A Pilot Study.The Laryngoscope · 2021Article
- Regenerative tissue filler for breast conserving surgery and other soft tissue restoration and reconstruction needs.Scientific reports · 2021Article
- Oligomeric collagen as an encapsulation material for islet/β-cell replacement: effect of islet source, dose, implant site, and administration format.American journal of physiology. Endocrinology and metabolism · 2020Article
- Tissue engineering applications in otolaryngology-The state of translation.Laryngoscope investigative otolaryngology · 2020Review
- Design and biofabrication of dermal regeneration scaffolds: role of oligomeric collagen fibril density and architecture.Regenerative medicine · 2020Article
- Advances in tissue engineering of peripheral nerve and tissue innervation - a systematic review.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveTissue engineering of the larynx requires a complex, multiple tissue layer design. Additionally, spontaneous reinnervation of the larynx after recurrent laryngeal nerve (RLN) injury is often disorganized, resulting in subpar function. This study investigates use of tissue-engineered cartilage and motor endplate-expressing (MEE) tissue-engineered skeletal muscle implants for laryngeal reconstruction and the promotion of organized reinnervation after RLN injury.
methodsF344 rat primary muscle progenitor cells (MPCs) were isolated. Three-dimensional muscle constructs were created by encapsulating MPCs in type I oligomeric collagen under passive tension. Constructs were then cultured in differentiation medium (MPC control constructs) or induced to form motor endplates (MEE constructs) with neurotrophic agents. Three-dimensional cartilage constructs were created with adipose stem cells differentiated in chondrocyte medium. The muscle and cartilage constructs were implanted into surgically created myochondral defects in the F344 rat larynx with injured or intact (control) RLN. At 1-, 3-, and 6-month timepoints, videolaryngoscopy, electromyography (EMG), histology, and immunohistochemistry were used to assess outcomes.
resultsAt all timepoints, cartilage-muscle implants were well integrated into host tissue. Functionally, there was increased vocal fold adduction and EMG activity in nerve-injured rats treated with the MEE constructs when compared to those treated with the MPC control constructs. Motor endplate-expressing constructs had increased myofiber cross-sectional area compared to MPC control constructs.
conclusionAlthough our laboratory previously demonstrated that muscle and cartilage constructs could be used separately for hemilaryngeal reconstruction, this study suggests combining them with the modification of MEEs rather than MPCs, resulting in improved muscle recovery after recurrent laryngeal nerve injury. LEVEL OF EVIDENCE: NA Laryngoscope, 129:1293-1300, 2019.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.