Evidence map›Paper›PMID 30547776›Full record

ArticleBMC veterinary research2018

Development and evaluation of a broadly reactive reverse transcription recombinase polymerase amplification assay for rapid detection of murine norovirus.

Lei Ma, Fanwen Zeng, Feng Cong, Bihong Huang, Yujun Zhu, Miaoli Wu, Fengjiao Xu, Wen Yuan, Ren Huang, Pengju Guo

Abstract readEvaluation Study
In one paragraph

Article in BMC veterinary research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Lei MaGuangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China.
Fanwen ZengCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, 510640, China.
Feng CongGuangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China.
Bihong HuangGuangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China.
Yujun ZhuGuangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China.
Miaoli WuGuangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China.
Fengjiao XuGuangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China.
Wen YuanGuangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China.
Ren HuangGuangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China.
Pengju GuoGuangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China. vetbio2016@hotmail.com.

Funding

Guangdong Science and Technology Department 2017A030303026;2017B030314171, 2017A070702001,2017A030303025; 2016A030303016Science and Technology Program of Guangzhou 201707010440
6 · The paper itself

Abstract

backgroundMurine norovirus (MNV) is recognized as the most prevalent viral pathogen in captive mouse colonies. The rapid detection assay for MNV would be a useful tool for monitoring and preventing MNV infection. A recombinase polymerase amplification (RPA) assay was established in this study to provide a solution for rapid and sensitive detection of MNV.

resultsThe detection limit of the RT-RPA assay for the detection of MNV was 1 × 10

conclusionsA broadly reactive RT-RPA assay was successfully established for MNV detection.

Indexed as

AnimalsAnimals, ZooCaliciviridae InfectionsMiceNorovirusNucleic Acid Amplification TechniquesRodent DiseasesSensitivity and SpecificityDetectionGenetic diversityMurine norovirusRecombinase polymerase amplification

Identifiers

PMID30547776
PMCPMC6295035

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.