Evidence map›Paper›PMID 30547283›Full record

ArticleIn vitro cellular & developmental biology. Animal2019

Pterostilbene exerts a protective effect via regulating tunicamycin-induced endoplasmic reticulum stress in mouse preimplantation embryos.

Obaid Ullah, Zhongshu Li, Ihsan Ali, Lijie Xu, Haixing Liu, Hua-Zhi Jin, Yan-Ya Fang, Qing-Guo Jin, Nanzhu Fang

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Examining the effect of activated cytotoxic (CD8Medical oncology (Northwood, London, England) · 2023
    Article
  3. The Beneficial Effects of Pterostilbene on Post-Thawed Bovine Spermatozoa.Animals : an open access journal from MDPI · 2023
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Obaid UllahDepartment of Animal Science, Agricultural College, Yanbian University, Yanji, 133002, Jilin, China.
Zhongshu LiDepartment of Animal Science, Agricultural College, Yanbian University, Yanji, 133002, Jilin, China.
Ihsan AliDepartment of Animal Science, Agricultural College, Yanbian University, Yanji, 133002, Jilin, China.
Lijie XuDepartment of Animal Science, Agricultural College, Yanbian University, Yanji, 133002, Jilin, China.
Haixing LiuDepartment of Animal Science, Agricultural College, Yanbian University, Yanji, 133002, Jilin, China.
Hua-Zhi JinDepartment of Animal Science, Agricultural College, Yanbian University, Yanji, 133002, Jilin, China.
Yan-Ya FangDepartment of Animal Science, Agricultural College, Yanbian University, Yanji, 133002, Jilin, China.
Qing-Guo JinDepartment of Animal Science, Agricultural College, Yanbian University, Yanji, 133002, Jilin, China. qgjin@ybu.edu.cn.
Nanzhu FangDepartment of Animal Science, Agricultural College, Yanbian University, Yanji, 133002, Jilin, China. nzfang@ybu.edu.cn.ORCID http://orcid.org/0000-0002-1185-3311
Yanbian University · CN

Funding

National Natural Science Foundation of China 31360546
6 · The paper itself

Abstract

Pterostilbene (PTS) mainly enriched in small fruits such as berries and grapes exerts an antioxidant effect. However, the protective effects of PTS against endoplasmic reticulum stress (ERS) have not yet been elucidated in mouse preimplantation embryo. ERS plays an important role in regulating the pathological and physiological processes, including embryonic development. We explored the protective effect of PTS on the tunicamycin (TM)-induced ERS in mouse preimplantation embryos. In vitro, culture medium was supplemented with different concentrations of TM and PTS. Our result indicated that treatment of zygotes with 0.5 μg/ml TM significantly decreased the development of day 4 blastocysts (P < 0.05), whereas 0.25 μM PTS supplementation improved the development rate of blastocysts. Moreover, TM treatment significantly increased (P < 0.05) the apoptotic index and reduced the total cell number of the blastocyst, whereas PTS treatment counteracted these effects. Additionally, TM potently increased expression levels of ERS-related proteins, such as GRP78, ATF6, PERK, p-Perk, IRE1, ATF4, and CHOP (P < 0.05). However, PTS and PTS + TM treatment decreased expression levels of ERS-related proteins (P < 0.05). Furthermore, expression level of the anti-apoptotic protein and gene BCL2 significantly decreased (P < 0.05) in TM-treated embryo but increased by PTS treatment (P < 0.05), whereas expression levels of the pro-apoptotic protein and gene BAX increased (P < 0.05) with TM but significantly decreased (P < 0.05) with co-treatment with PTS. In summary, PTS treatment significantly increased the development potential of mouse embryo by reduction of ERS.

Indexed as

AnimalsApoptosisBlastocystEndoplasmic Reticulum Chaperone BiPEndoplasmic Reticulum StressFemaleGene Expression Regulation, DevelopmentalGlutathioneMicePhosphorylationProtective AgentsProto-Oncogene Proteins c-bcl-2Reactive Oxygen SpeciesSignal TransductionStilbenesTranscription FactorsEndoplasmic Reticulum Chaperone BiPGlutathioneHspa5 protein, mouseProtective AgentsProto-Oncogene Proteins c-bcl-2pterostilbeneReactive Oxygen SpeciesStilbenesTranscription FactorsTunicamycinEndoplasmic reticulum stressIn vitro culture mediumMouse embryoPterostilbeneTunicamycin

Identifiers

PMID30547283
OpenAlexW2905018692

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.