Evidence map›Paper›PMID 30545311›Full record

ArticleBMC genomics2018

Identification and characteristics of muscle growth-related microRNA in the Pacific abalone, Haliotis discus hannai.

Jianfang Huang, Xuan Luo, Miaoqin Huang, Guangmou Liu, Weiwei You, Caihuan Ke

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Jianfang HuangState Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361102, China.
Xuan LuoState Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361102, China.
Miaoqin HuangState Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361102, China.
Guangmou LiuNational Engineering Research Center of Marine Shellfish, Weihai, 264316, China.
Weiwei YouState Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361102, China. wwyou@xmu.edu.cn.
Caihuan KeState Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361102, China. chke@xmu.edu.cn.
Xiamen University · CN

Funding

Earmarked Fund for Modern Agro-industry Technology Research System No. CARS-49Key S & T Program of Shandong Province No. 2016GGH4513National Natural Science Foundation of China 31872564National Natural Science Foundation of China No.U1605213Xiamen Southern Oceanographic Center No. 18GZY012HJ02
6 · The paper itself

Abstract

backgroundThe Pacific abalone, Haliotis discus hannai, is the most important cultivated abalone in China. Improving abalone muscle growth and increasing the rate of growth are important genetic improvement programs in this industry. MicroRNAs are important small noncoding RNA molecules that regulate post-transcription gene expression. However, no miRNAs have been reported to regulate muscle growth in H. discus hannai.

resultswe profiled six small RNA libraries for three large abalone individuals (L_HD group) and three small individuals (S_HD group) using RNA sequencing technology. A total of 205 miRNAs, including 200 novel and 5 known miRNAs, were identified. In the L_HD group, 3 miRNAs were up-regulated and 7 were down-regulated compared to the S_HD specimens. Bioinformatics analysis of miRNA target genes revealed that miRNAs participated in the regulation of cellular metabolic processes, the regulation of biological processes, the Wnt signaling pathway, ECM-receptor interaction, and the MAPK signaling pathway, which are associated with regulating growth. Bone morphogenetic protein 7 (BMP7) was verified as a target gene of hdh-miR-1984 by a luciferase reporter assay and we examined the expression pattern in different developmental stages.

conclusionThis is the first study to demonstrate that miRNAs are related to the muscle growth of H. discus hannai. This information could be used to study the mechanisms of abalone muscle growth. These DE-miRNAs may be useful as molecular markers for functional genomics and breeding research in abalone and closely related species.

Indexed as

3' Untranslated RegionsAnimalsAntagomirsBase SequenceBone Morphogenetic Protein 7Cluster AnalysisComputational BiologyGastropodaGene Expression RegulationGene LibraryGene Regulatory NetworksMicroRNAsMuscle DevelopmentMusclesRNASequence Alignment3' Untranslated RegionsAntagomirsBone Morphogenetic Protein 7MicroRNAsRNAHaliotis discus hannaiHdh-miR-1984miRNAMuscle growthPacific abalone

Identifiers

PMID30545311
PMCPMC6293614
OpenAlexW2904798570

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.