Evidence map›Paper›PMID 30544986›Full record

ReviewGenes2018

DNA-Based Super-Resolution Microscopy: DNA-PAINT.

Daniel J Nieves, Katharina Gaus, Matthew A B Baker

Abstract readReview
In one paragraph

Review in Genes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Review
  11. Article
  12. Review
  13. Article
  14. Article
  15. Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging.Angewandte Chemie (International ed. in English) · 2023
    Article
  16. Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2023
    Article
  17. Choosing the Probe for Single-Molecule Fluorescence Microscopy.International journal of molecular sciences · 2022
    Review
  18. Article
  19. Article
  20. 21 Fluorescent Protein-Based DNA Staining Dyes.Molecules (Basel, Switzerland) · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Daniel J NievesEMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia. d.nieves@unsw.edu.au.ORCID 0000-0002-0873-4418
Katharina GausEMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia. k.gaus@unsw.edu.au.
Matthew A B BakerSchool of Biotechnology and Biomolecular Science, University of New South Wales, Sydney, NSW 2052, Australia. matthew.baker@unsw.edu.au.

Funding

University of New South Wales Scientia Research Fellowship
6 · The paper itself

Abstract

Super-resolution microscopies, such as single molecule localization microscopy (SMLM), allow the visualization of biomolecules at the nanoscale. The requirement to observe molecules multiple times during an acquisition has pushed the field to explore methods that allow the binding of a fluorophore to a target. This binding is then used to build an image via points accumulation for imaging nanoscale topography (PAINT), which relies on the stochastic binding of a fluorescent ligand instead of the stochastic photo-activation of a permanently bound fluorophore. Recently, systems that use DNA to achieve repeated, transient binding for PAINT imaging have become the cutting edge in SMLM. Here, we review the history of PAINT imaging, with a particular focus on the development of DNA-PAINT. We outline the different variations of DNA-PAINT and their applications for imaging of both DNA origamis and cellular proteins via SMLM. Finally, we reflect on the current challenges for DNA-PAINT imaging going forward.

Indexed as

DNADNA origamiDNA PAINTfluorescence microscopySMLMsuper-resolution microscopy

Identifiers

PMID30544986
PMCPMC6315775

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.